{"product_id":"rat-primary-urinary-bladder-smooth-muscle-cells-bhc10901909","title":"Rat Primary Urinary Bladder Smooth Muscle Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRat Primary Urinary Bladder Smooth Muscle Cells are isolated from healthy rat bladder tissue and exhibit the characteristic spindle-shaped morphology and contractile phenotype of smooth muscle cells. These cells provide a physiologically relevant in vitro model for studying bladder smooth muscle function, contraction, tissue remodeling, and mechanisms underlying urinary tract disorders such as bladder dysfunction and overactive bladder. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Rat (R. norvegicus)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Kidney\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, multipolar\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding guidance:\u003c\/strong\u003e 6,000 - 10,000 cells\/cm²\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Human Smooth Muscle Cell Growth Medium Kit (TM108) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eSmooth muscle cells regulate vessel tone, extracellular matrix remodeling, and responses to growth factors, cytokines, and mechanical cues. In culture, they are often used to study contractile-to-synthetic state transitions.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStudies frequently examine how inflammatory, mechanical, or metabolic cues alter smooth muscle phenotype and matrix production.\u003c\/li\u003e\n\u003cli\u003ePrimary vascular smooth muscle cells are used to model remodeling pathways relevant to stenosis, calcification, and vascular injury.\u003c\/li\u003e\n\u003cli\u003eComparative donor and vessel-source studies help capture regional heterogeneity across the arterial tree.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMeasure contractile marker expression or phenotype switching after defined perturbations.\u003c\/li\u003e\n\u003cli\u003eEvaluate proliferation, migration, and matrix-remodeling responses in vascular remodeling models.\u003c\/li\u003e\n\u003cli\u003eUse in co-culture with endothelial cells or fibroblasts to examine vessel wall signaling.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Human Smooth Muscle Cell Growth Medium Kit (TM108) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 6,000 - 10,000\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10901909\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195753619821,"sku":"T4937","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/gGclXQd6KM7PkU1Ykk6frU6clmBYbwSFMViHXcVu.png?v=1775322483","url":"https:\/\/www.ebiohippo.com\/products\/rat-primary-urinary-bladder-smooth-muscle-cells-bhc10901909","provider":"BioHippo","version":"1.0","type":"link"}