{"product_id":"rat-solute-carrier-family-12-member-4-slc12a4-elisa-kit-bhe11401715","title":"Rat Solute carrier family 12 member 4 (SLC12A4) ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n\u003c\/style\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eThe full-length sequence was obtained from EST clones and by RT-PCR of HEK293 cell RNA. The 1,085-amino acid KCC1 protein is 24 to 25% identical to NKCC1 and SLC12A3 and shares 97% identity with rabbit KCC1. The overall structure of KCC1 is similar to that of other cation-chloride cotransporters, with 12 predicted transmembrane regions, a large extracellular loop with potential N-linked glycosylation sites, and cytoplasmic N- and C-terminal domains. Northern blot analysis revealed that KCC1 is expressed ubiquitously. KCC1 exhibits the functional properties of the red cell K-Cl cotransporter, i.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThis assay employs a two-site sandwich ELISA to quantitate SLC12A4 in samples. An antibody specific for SLC12A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC12A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC12A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC12A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\n\u003ch2\u003ePerformance Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e0.127 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e0.312-20 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTotal Assay Time\u003c\/th\u003e\n\u003ctd\u003e3.5–5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCompatible Sample Types\u003c\/th\u003e\n\u003ctd\u003eserum, plasma, cell culture supernatant, tissue homogenate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (TMB\/HRP)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e4°C (short-term); -20°C (long-term)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003e✓ Research-Grade Validation\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only (RUO).\u003c\/strong\u003e This product is intended for research purposes only and is not approved for diagnostic, therapeutic, or clinical use.\u003c\/div\u003e\n\u003cp\u003eHandle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Wuhan Abebio Science Co Ltd","offers":[{"title":"48 T","offer_id":53337695748461,"sku":"AE19218RA-48T","price":325.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53337830097261,"sku":"AE19218RA-96T","price":610.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_2236fed6-95a3-4ab1-96e0-335996c270f2.jpg?v=1778950092","url":"https:\/\/www.ebiohippo.com\/products\/rat-solute-carrier-family-12-member-4-slc12a4-elisa-kit-bhe11401715","provider":"BioHippo","version":"1.0","type":"link"}