{"product_id":"rb1-antibody-retinoblastoma-bha17135458","title":"RB1 Antibody \/ Retinoblastoma","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRB1 Antibody \/ Retinoblastoma is a anti-RB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RB1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRB1 antibody detects Retinoblastoma-associated protein, a pivotal tumor suppressor that controls cell cycle progression, differentiation, and genome stability. RB1 is the founding member of the retinoblastoma protein family, which also includes RBL1 (p107) and RBL2 (p130). Through regulation of E2F transcription factors, RB1 governs the G1-to-S phase transition, making it one of the most important gatekeepers of cellular proliferation. The RB1 antibody is widely used in cancer biology, developmental research, and cell cycle studies.\u003cbr\u003e\u003cbr\u003eRB1 is encoded by the RB1 gene located on human chromosome 13q14.2. The protein is approximately 110 kilodaltons and composed of an N-terminal domain, a central pocket domain responsible for E2F binding, and a C-terminal regulatory region. RB1 activity is primarily modulated through phosphorylation by cyclin-dependent kinases (CDKs). Hypophosphorylated RB1 binds and inhibits E2F transcription factors, repressing transcription of S-phase genes. Upon phosphorylation, RB1 releases E2Fs, allowing cell cycle progression.\u003cbr\u003e\u003cbr\u003eThe RB1 antibody detects the 110 kilodalton protein and phospho-variants by western blot and demonstrates nuclear localization in immunocytochemistry. RB1 serves as a platform for chromatin modifiers, recruiting histone deacetylases (HDACs) and methyltransferases to silence gene expression during quiescence and differentiation. In addition to cell cycle control, RB1 promotes genomic stability by coordinating DNA repair, replication fidelity, and mitotic checkpoints.\u003cbr\u003e\u003cbr\u003eLoss or mutation of RB1 is a hallmark of numerous cancers, including retinoblastoma, osteosarcoma, and small-cell lung carcinoma. RB1 inactivation leads to unchecked E2F activity, excessive proliferation, and impaired DNA repair. Conversely, reactivation of RB1 signaling can suppress tumor growth and restore differentiation potential in malignant cells.\u003cbr\u003e\u003cbr\u003eBeyond oncology, RB1 regulates tissue-specific differentiation, especially in muscle and neuronal lineages, by interacting with lineage-determining transcription factors. Its phosphorylation status serves as an indicator of cell cycle activity and is frequently used as a diagnostic biomarker.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RB1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RB1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RB1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RB1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RB1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292690797,"sku":"FY12555","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fa6ab181-89de-409e-96d4-612b6aa1d15a.jpg?v=1772019311","url":"https:\/\/www.ebiohippo.com\/products\/rb1-antibody-retinoblastoma-bha17135458","provider":"BioHippo","version":"1.0","type":"link"}