{"product_id":"rbck1-antibody-ranbp-type-and-c3hc4-type-zinc-finger-containing-protein-1-bha17135379","title":"RBCK1 Antibody \/ RanBP-type and C3HC4-type zinc finger-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRBCK1 Antibody \/ RanBP-type and C3HC4-type zinc finger-containing protein 1 is a anti-RBCK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RBCK1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eRBCK1 antibody recognizes RanBP-type and C3HC4-type zinc finger-containing protein 1, an E3 ubiquitin-protein ligase involved in inflammation, innate immunity, and linear ubiquitin chain assembly. RBCK1 belongs to the HOIL-1 subfamily of RBR (RING-between-RING) E3 ligases and functions as part of the linear ubiquitin chain assembly complex (LUBAC) together with HOIP (RNF31) and SHARPIN. This complex is essential for NF-kappaB activation and regulation of immune responses. The RBCK1 antibody is widely used in immunology, cell signaling, and inflammation research to study ubiquitin-dependent regulatory mechanisms governing cell survival and immune homeostasis.\u003cbr\u003e\u003cbr\u003eRBCK1 is encoded by the RBCK1 gene located on human chromosome 20q11.22. The protein contains several domains, including an N-terminal ubiquitin-like (UBL) domain, a RanBP-type zinc finger, a central RBR domain, and a C-terminal NZF-type zinc finger. These motifs enable RBCK1 to interact with ubiquitin, E2 conjugating enzymes, and adaptor proteins to assemble linear and lysine-63-linked ubiquitin chains. Through these modifications, RBCK1 promotes activation of NF-kappaB signaling and contributes to the regulation of cytokine production and cell survival following immune receptor stimulation.\u003cbr\u003e\u003cbr\u003eThe RBCK1 antibody is useful for detecting the 56-60 kilodalton protein by western blot and immunoprecipitation. In cells, RBCK1 localizes to the cytoplasm and occasionally to the nucleus, depending on signaling state. Functional studies have shown that deficiency or mutation of RBCK1 leads to defects in LUBAC function, resulting in impaired immune signaling, chronic inflammation, or autoinflammatory disorders such as polyglucosan body myopathy. Loss of RBCK1 function also compromises the stability of HOIP, reducing linear ubiquitination of NEMO and other substrates critical for inflammatory gene transcription.\u003cbr\u003e\u003cbr\u003eBeyond immunity, RBCK1 participates in protein quality control through the degradation of misfolded cytosolic proteins. It contributes to the ubiquitin-proteasome system and has been implicated in muscle physiology and mitochondrial regulation. Recent studies suggest that RBCK1 also influences cellular metabolism and redox balance, linking ubiquitin signaling to broader physiological processes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RBCK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RBCK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RBCK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RBCK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RBCK1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290036589,"sku":"FY12476","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_70b52fc0-6fd8-4757-b8f8-751f650009e6.jpg?v=1782237006","url":"https:\/\/www.ebiohippo.com\/products\/rbck1-antibody-ranbp-type-and-c3hc4-type-zinc-finger-containing-protein-1-bha17135379","provider":"BioHippo","version":"1.0","type":"link"}