{"product_id":"recombinant-5hmc-antibody-bha17103732","title":"Recombinant 5hmC Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e 5hmC antibody supplied as a purified reagent for DB, hMeDIP, ICC, IHC, ELISA in Human samples. This product is a Recombinant rabbit monoclonal antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e  Rabbit Monoclonal; host Rabbit; isotype Rabbit IgG; clone RM236.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein A purified from animal origin-free supernatant.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e DB, hMeDIP, ICC, IHC, ELISA.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this  5hmC antibody..\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e 5hmC is the intended antigen for this primary antibody. Reported biological context includes: This  5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e\n  \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDB: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e\n  \u003cli\u003ehMeDIP: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e\n  \u003cli\u003eImmunocytochemistry (ICC): visualize intracellular distribution and morphology-linked changes in cultured cells.\u003c\/li\u003e\n  \u003cli\u003eImmunohistochemistry (IHC): profile tissue and cell-type distribution in fixed specimens and evaluate spatial heterogeneity in expression.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e\n  \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e\n  \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- NCBI Gene search ( 5hmC) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=+5hmC\n- Ensembl search ( 5hmC) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=+5hmC\n- PubMed search ( 5hmC) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=+5hmC\n- Reactome pathway search ( 5hmC) — Reactome — https:\/\/reactome.org\/content\/query?q=+5hmC\n--\u003e","brand":"NSJ Bioreagents","offers":[{"title":"1 mg\/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 50 ug","offer_id":53043680674157,"sku":"R20246-50UG","price":530.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5436da8d-36b4-4f54-92a9-0ab7d17edbec.jpg?v=1771934404","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-5hmc-antibody-bha17103732","provider":"BioHippo","version":"1.0","type":"link"}