{"product_id":"recombinant-akt-antibody-ph-domain-bha17103816","title":"Recombinant AKT Antibody \/ PH domain","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e AKT antibody supplied as a purified reagent for WB, IHC-P in Human samples. This product is a Recombinant rabbit monoclonal antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only. The target is commonly annotated with cytoplasmic, membranous, nuclear localization context, which may inform staining patterns.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e  Rabbit Monoclonal; host Rabbit; isotype Rabbit IgG; clone RM316.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein A purified from animal origin-free supernatant.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB, IHC-P.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A peptide corresponding to the PH domain of human AKT1\/2\/3 was used as the immunogen for the  AKT antibody..\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Cytoplasmic, membranous, nuclear (annotation-level guidance; cell state and isoforms can shift patterns).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e AKT is the intended antigen for this primary antibody. Reported biological context includes: Akt isoforms are overexpressed in a variety of human tumors, and, at the genomic level, are amplified in gastric adenocarcinomas (Akt1), ovarian (Akt2), pancreatic (Akt2) and breast (Akt2) cancer. The role of Akt3 is less clear, though it appears to be predominantly expressed in the brain.\u003cbr\u003e\u003cbr\u003eAkt possesses a protein domain known as a PH domain, or Pleckstrin Homology domain, named after Pleckstrin, the protein in which it was first discovered. Subcellular localization information (Cytoplasmic, membranous, nuclear) can be useful when interpreting IF\/ICC patterns and selecting compartment-enriched lysates for WB.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePost-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.\u003c\/li\u003e\n  \u003cli\u003eSignal-flow and turnover studies: researchers pair immunodetection with perturbations that modulate enzymatic activity or proteostasis to understand regulation, stability, and feedback.\u003c\/li\u003e\n  \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e\n  \u003cli\u003eIHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e\n  \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e\n  \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry (P31749, P31751, Q9Y243) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P31749, P31751, Q9Y243\/entry\n- NCBI Gene search ( AKT) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=+AKT\n- Ensembl search ( AKT) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=+AKT\n- PubMed search ( AKT) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=+AKT\n- Reactome pathway search ( AKT) — Reactome — https:\/\/reactome.org\/content\/query?q=+AKT\n--\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 100 ul","offer_id":53043684311405,"sku":"R20335-0.1ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c9a0566d-bbf2-4f34-aaa8-9cf2ef7f3f37.jpg?v=1771934423","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-akt-antibody-ph-domain-bha17103816","provider":"BioHippo","version":"1.0","type":"link"}