{"product_id":"recombinant-apis-mellifera-melittin-melt-bhp10507488","title":"Recombinant Apis mellifera Melittin (MELT)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eMELT\u003c\/strong\u003e from Apis mellifera (Honeybee), produced in E.coli (region 44-69aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 44-69aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as MEL (MLT) (Allergen Api m 3) (Allergen Api m III) (Allergen: Api m 4). Melittin: Main toxin of bee venom with strong hemolytic activity and antimicrobial activity . It has enhancing effects on bee venom phospholipase A2 activity . This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis . It acts as a voltage-gated pore with higher selectivity for anions over cations . The ion conductance has been shown to be voltage-dependent . Self-association of melittin in membranes is promoted by high ionic strength, but not by the presence of negatively charged lipids . In vivo, intradermal injection into healthy human volunteers produce sharp pain sensation and an inflammatory response . It produces pain by activating primary nociceptor cells directly and indirectly due to its ability to activate plasma membrane phospholipase A2 and its pore-forming activity .\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eMelittin: Main toxin of bee venom with strong hemolytic activity and antimicrobial activity . It has enhancing effects on bee venom phospholipase A2 activity . This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis . It acts as a voltage-gated pore with higher selectivity for anions over cations . The ion conductance has been shown to be voltage-dependent . Self-association of melittin in membranes is promoted by high ionic strength, but not by the presence of negatively charged lipids . In vivo, intradermal injection into healthy human volunteers produce sharp pain sensation and an inflammatory response . It produces pain by activating primary nociceptor cells directly and indirectly due to its ability to activate plasma membrane phospholipase A2 and its pore-forming activity .\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving MELT\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P01501 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P01501 - NCBI Gene search: MELT — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MELT - Ensembl search: MELT — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MELT - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053415260525,"sku":"CSB-EP355665DNK-1MG","price":2632.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053550002541,"sku":"CSB-EP355665DNK-100UG","price":895.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053550035309,"sku":"CSB-EP355665DNK-20UG","price":554.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP355665DNK-SDS.jpg?v=1772177676","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-apis-mellifera-melittin-melt-bhp10507488","provider":"BioHippo","version":"1.0","type":"link"}