{"product_id":"recombinant-bacillus-subtilis-subsp-natto-subtilisin-nat-aprn-bhp10508078","title":"Recombinant Bacillus subtilis subsp. natto Subtilisin NAT (aprN)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eaprN\u003c\/strong\u003e from Bacillus subtilis subsp. natto, produced in E.coli (region 107-381aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 107-381aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Nattokinase. Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity. Miscellaneous Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation. In Japan this enzyme, isolated from a process of fermentation involving boiled soybeans and Bacillus natto, is used as a food supplement because it is reported to reduce and prevent blood clotting. Catalytic activityi Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. EC:3.4.21.62.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eSubtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity. Miscellaneous Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation. In Japan this enzyme, isolated from a process of fermentation involving boiled soybeans and Bacillus natto, is used as a food supplement because it is reported to reduce and prevent blood clotting. Catalytic activityi Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. EC:3.4.21.62\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving aprN\u003c\/li\u003e\n\u003cli\u003eEnzymatic activity measurements with defined substrates\/cofactors (assay-dependent)\u003c\/li\u003e\n\u003cli\u003eCompound screening using concentration–response concepts (assay-dependent)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P35835 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P35835 - NCBI Gene search: aprN — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=aprN - Ensembl search: aprN — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=aprN - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053433971053,"sku":"CSB-EP336091BRL-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053587292525,"sku":"CSB-EP336091BRL-100UG","price":729.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053587325293,"sku":"CSB-EP336091BRL-20UG","price":388.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP336091BRL-SDS.jpg?v=1772177744","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-bacillus-subtilis-subsp-natto-subtilisin-nat-aprn-bhp10508078","provider":"BioHippo","version":"1.0","type":"link"}