{"product_id":"recombinant-bacterial-outer-membrane-protein-a-bhp11004585","title":"Recombinant Bacterial Outer Membrane Protein-A","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Bacterial Outer Membrane Protein-A\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eThe OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A.salmonicida, Shigella dysenteriae and E.coli.OmpA’s major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity, as well as a role in conjugation and bacteriophage binding.Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease.Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein.The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E.coli BL21(DE3). The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein.Recombinant A-protein was compared by biochemical, immunological and molecular methods with the A-protein isolated from atypical A.salmonicida bacterial cells by the glycine and the membrane extraction methods.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"100 ug","offer_id":53038150910317,"sku":"pro-571-100UG","price":500.0,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53038482227565,"sku":"pro-571-200UG","price":700.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53038482260333,"sku":"pro-571-1MG","price":2700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-outer-membrane-protein-a-PRO-571.png?v=1782158334","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-bacterial-outer-membrane-protein-a-bhp11004585","provider":"BioHippo","version":"1.0","type":"link"}