{"product_id":"recombinant-bothrops-jararaca-zinc-metalloproteinase-disintegrin-like-botrocetin-partial-bhp10508083","title":"Recombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003ebotrocetin\u003c\/strong\u003e from Bothrops jararaca (Jararaca) (Bothrops jajaraca), produced in E.coli (region 1-25aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 1-25aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Snake venom metalloproteinase von Willebrand factor-dependent platelet coagglutinin. Metalloproteinase that binds to von Willebrand factor (VWF) and induces its interaction with GPIb (GP1BA), resulting in platelet aggregation. Miscellaneous There are two distinct forms of the vWF-dependent platelet coagglutinin. The dimeric form (snaclec) is 34-fold more active than the metalloprotease botrocetin in promoting vWF binding to platelets (PubMed:1993206).\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eMetalloproteinase that binds to von Willebrand factor (VWF) and induces its interaction with GPIb (GP1BA), resulting in platelet aggregation. Miscellaneous There are two distinct forms of the vWF-dependent platelet coagglutinin. The dimeric form (snaclec) is 34-fold more active than the metalloprotease botrocetin in promoting vWF binding to platelets (PubMed:1993206).\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving botrocetin\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P22028 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P22028 - NCBI Gene search: botrocetin — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=botrocetin - Ensembl search: botrocetin — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=botrocetin - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053433217389,"sku":"CSB-EP325199BUW-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053587882349,"sku":"CSB-EP325199BUW-100UG","price":729.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053587915117,"sku":"CSB-EP325199BUW-20UG","price":388.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP325199BUW-SDS.jpg?v=1772177746","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-bothrops-jararaca-zinc-metalloproteinase-disintegrin-like-botrocetin-partial-bhp10508083","provider":"BioHippo","version":"1.0","type":"link"}