{"product_id":"recombinant-bovine-coronavirus-non-structural-protein-2a-2a-bhp10509397","title":"Recombinant Bovine coronavirus Non-structural protein 2a (2a)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eRecombinant Bovine coronavirus Non-structural protein 2a (2a) is a recombinant protein reagent derived from Bovine coronavirus (strain LY-138) (BCoV) (BCV) and produced in Baculovirus. It is commonly used to support Microbiology research by enabling binding assays, assay development and protein–protein interaction studies in controlled in vitro settings.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 1-278aa. Region selection can focus on functional domains, improve solubility, or isolate interaction surfaces for targeted studies.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Baculovirus. Expression host can influence folding and the presence\/absence of post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eTag \/ fusion:\u003c\/strong\u003e C-terminal 6xHis-tagged. Tags can support purification and detection; evaluate potential tag effects when studying sensitive interactions.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight (reported):\u003c\/strong\u003e 37.7 kDa. Apparent size may vary with tags, processing, and gel conditions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWhen comparing results across batches or platforms, interpret signals in the context of construct design (region, tags) and expression host, especially for modification-dependent interactions.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eThe gene commonly associated with this target is \u003cstrong\u003e2a\u003c\/strong\u003e. BCoV-NSP2A refers to a protein target that is studied across multiple biological contexts; annotations and nomenclature can vary by species and isoform. This product corresponds to the Bovine coronavirus (strain LY-138) (BCoV) (BCV) sequence context, which can be important when comparing homologs or orthologs across model systems. For curated functional annotations, domains, and sequence features, consult primary databases (e.g., UniProt\/NCBI) and the recent literature for the specific organism and isoform.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eUsing recombinant proteins to enable quantitative binding measurements and reagent benchmarking.\u003c\/li\u003e \u003cli\u003eStudying domain- and isoform-specific effects in pathway models and interaction networks.\u003c\/li\u003e \u003cli\u003eDeveloping robust, reproducible assays that connect molecular readouts to cellular phenotypes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAssay and standard development for immunoassays or binding-based detection methods.\u003c\/li\u003e \u003cli\u003eProtein–protein interaction studies (e.g., receptor–ligand or complex assembly) using purified components.\u003c\/li\u003e \u003cli\u003eStructure–function analysis, including domain mapping or evaluation of sequence variants.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eIn quantitative assay development, changes in binding or activity readouts are typically interpreted relative to appropriate negative\/positive controls and, where possible, orthogonal assay formats that support the same conclusion.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eRecombinant constructs may represent a defined region (domain) rather than the full-length protein; interpret results in the context of the expressed region.\u003c\/li\u003e \u003cli\u003eTag or fusion elements can aid purification and detection but may influence binding surfaces or oligomerization; consider tag controls when relevant.\u003c\/li\u003e \u003cli\u003eSpecies and isoform differences can affect interaction partners and post-translational modifications; align experimental controls to the intended biological context.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry for Q9QAS3 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q9QAS3\/entry - NCBI Gene search (2a) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=2a - PubMed search (2a) — NCBI — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=2a - RCSB PDB search (2a) — RCSB PDB — https:\/\/www.rcsb.org\/search?query=2a - PubMed search (BCoV-NSP2A) — NCBI — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BCoV-NSP2A --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53065286582637,"sku":"CSB-BP889517BJJ-1MG","price":2240.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53065426076013,"sku":"CSB-BP889517BJJ-100UG","price":887.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53065426108781,"sku":"CSB-BP889517BJJ-20UG","price":317.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-BP889517BJJ-SDS.jpg?v=1772476447","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-bovine-coronavirus-non-structural-protein-2a-2a-bhp10509397","provider":"BioHippo","version":"1.0","type":"link"}