{"product_id":"recombinant-bovine-enterokinase-his-expressed-in-yeast-5-u-mu-l-bhz20800026","title":"Recombinant Bovine Enterokinase, His, Expressed in Yeast (5 U\/μL)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRecombinant \u003c\/span\u003e\u003cspan\u003eE\u003c\/span\u003e\u003cspan\u003enterokinase\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis a high-purity recombinant fragment of bovine enterokinase light chain, with an amino acid sequence identical to that of the bovine enterokinase light chain. It has the same specific cleavage site as naturally extracted enterokinase, which is Asp-Asp-Asp-Asp-Lys (DDDDK). It can be used to remove fusion proteins located at the N-terminus of proteins to remove unwanted fusion tags, ensuring the accurate N-terminal sequence of recombinant fusion proteins. At the same time, Recombinant \u003c\/span\u003e\u003cspan\u003eE\u003c\/span\u003e\u003cspan\u003enterokinase has higher cutting activity than natural enzymes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eR\u003c\/span\u003e\u003cspan\u003eecombinant\u003c\/span\u003e\u003cspan\u003e E\u003c\/span\u003e\u003cspan\u003enterokinase\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003eHis\u003c\/span\u003e\u003cspan\u003e Tag \u003c\/span\u003e\u003cspan\u003eis a high-purity, high-activity, and highly specific bovine enterokinase expressed by Pichia pastoris secretion. It can effectively cleave fusion proteins within a wide pH range (4.5-9.5) and a wide temperature range, and still has partial activity under various detergents and denaturants. This product has a His tag, which can be easily removed through Ni\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e affinity column after the cutting reaction, greatly simplifying the subsequent purification process.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eStrong specificity\u003c\/strong\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e—\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eA specific protease\u003c\/span\u003e\u003cspan\u003e that \u003c\/span\u003e\u003cspan\u003ecut\u003c\/span\u003e\u003cspan\u003es the\u003c\/span\u003e\u003cspan\u003e carboxyl termi\u003c\/span\u003e\u003cspan\u003enus\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eof lysine\u003c\/span\u003e\u003cspan\u003e contain\u003c\/span\u003e\u003cspan\u003eing\u003c\/span\u003e\u003cspan\u003e four aspartic acid\u003c\/span\u003e\u003cspan\u003es in front\u003c\/span\u003e\u003cspan\u003e: Asp-Asp-Asp-Asp-Lys\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eH\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003eigh purity\u003c\/strong\u003e—\u003c\/span\u003e\u003cspan\u003eNo \u003c\/span\u003e\u003cspan\u003eother \u003c\/span\u003e\u003cspan\u003eprotease,\u003c\/span\u003e\u003cspan\u003eNo non-specific cutting\u003c\/span\u003e\u003cspan\u003e. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eAnimal free\u003c\/strong\u003e—\u003cspan\u003eRecombinantly produced, free from exogenous viral contamination, and no animal-derived materials are used in the production process.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cstrong\u003eQuality Stability:\u003c\/strong\u003e Batch production ensures stable and continuous batch manufacturing; there are no differences between product batches, ensuring consistent quality.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cstrong\u003eAdequate Capacity:\u003c\/strong\u003e Yeasen possesses fermenters ranging from 5L to 1500L, meeting the varying batch requirements of different clients at different stages.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eIn the production process of fusion peptides and proteins, it is used to remove fusion proteins located at the N-terminus of proteins to remove unwanted fusion tags, ensuring the accurate N-terminal sequence of recombinant fusion proteins.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eIn the field of biopharmaceuticals, the production and preparation of GLP-1 analog peptide drugs are commonly used.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" class=\"MsoTableGrid\" style=\"width: 99.9965%; height: 467.525px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eYeast recombinant expression\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMolecular Weight*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTheoretical value 22.7 kDa\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAppearance\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSterile liquid\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.0469px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 58.0469px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStorage Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 58.0469px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 mM Tris-HCl, 250 mM NaCl, 2 mM CaCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 50% Glycerol, pH 8.0\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEnzyme Concentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 U\/μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5859px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 35.5859px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 35.5859px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e≥95%\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 231.549px;\"\u003e\n\u003ctd style=\"width: 35.5637%; height: 231.549px;\" valign=\"center\" width=\"25.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eActivity Definition*\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 64.3628%; height: 231.549px;\" valign=\"top\" width=\"74.0800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eOne unit of activity is defined as the amount of enzyme required to cut 95% of the 50 μg fusion protein with enterokinase cleavage site (Enterokinase Positive Substrate, molecular weight approximately 64.6 kDa, Cat#20391ES) under the condition of 25°C, 12-16 hours, in a buffer system (20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2, pH 8.0).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong\u003eMolecular Weight: \u003c\/strong\u003e*Due to the glycosylation effect after expression in Pichia pastoris, the molecular weight of the target protein shown by SDS-PAGE is about 40 kDa.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eActivity Definition\u003cspan\u003e：\u003c\/span\u003e\u003c\/strong\u003e*The cleavage efficiency may vary depending on the substrate. Generally, smaller proteins tend to have higher cleavage efficiency. The enzyme activity defined by Yeasen is based on a large protein with a molecular weight of approximately 64.6 kDa. For oligopeptide substrates, it can generally reach a maximum of 1 unit to cleave 500 μg.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"144\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"114\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"124\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES76\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"114\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES90\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"118\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES92\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"117\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395ES94\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e20395\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"144\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\" style=\"text-align: left;\"\u003e\u003cspan\u003eRecombinant Enterokinase, His Tag\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"114\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ca name=\"OLE_LINK5\"\u003e\u003c\/a\u003e100 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"124\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e500 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"114\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e5000 U\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"118\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e100 KU\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"117\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e1 MU\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan\u003e（\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e1000 KU\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eStorage \u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe product can be stored at \u003c\/span\u003e\u003cspan\u003e-25\u003c\/span\u003e\u003cspan\u003e ~ \u003c\/span\u003e\u003cspan\u003e-15\u003c\/span\u003e℃ for 2 years.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eFigure\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e1. Enterokinase activity test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20395-1.jpg?v=1750754147\"\u003e\u003c\/div\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003eFigure 1. The enzyme cutting effect of Yeasen enterokinase (Cat#20395ES) is consistent with Supplier N*\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e[Note]: \u003c\/strong\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/products\/20391?_pos=1\u0026amp;_psq=20391\u0026amp;_ss=e\u0026amp;_v=1.0\"\u003e\u003cu\u003e\u003cspan class=\"16\"\u003eThe substrate (\u003c\/span\u003e\u003c\/u\u003e\u003cu\u003e\u003cspan class=\"16\"\u003eCat#\u003c\/span\u003e\u003c\/u\u003e\u003cu\u003e\u003cspan class=\"16\"\u003e20391ES)\u003c\/span\u003e\u003c\/u\u003e\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e is a fusion protein composed of two specific protein sequences connected by DDDDK (enterokinase recognition and cutting site), with an overall molecular weight of about 64.6 kDa, which can be specifically cut into two independent protein fragments by enterokinase, with molecular weights of about 27.9 kDa and 36.6 kDa, respectively. This product, as an enterokinase substrate, can be used for semi-quantitative or qualitative enzyme activity detection of recombinant or natural enterokinase.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e2. Enterokinase purity test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_40f713c0-8139-4916-8277-37ee0cd9248f.png?v=1750315648\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cb\u003eFigure 2. The purity of enterokinase (\u003c\/b\u003e\u003cb\u003eCat#\u003c\/b\u003e\u003cb\u003e20395ES) is greater than 95%\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e[Note]: \u003c\/strong\u003eThe theoretical value of enterokinase is 22.7 kDa. Due to the glycosylation effect after expression in Pichia pastoris, the SDS-PAGE shows that the molecular weight of the target protein is about 40 kDa. Generally, the glycosylated bands will have a slightly diffuse band running upwards (as shown in the right figure). Our company can also provide deglycosylation enzyme Endo H (Cat#20414) for customers to deglycosylate and run gel to measure purity (as shown in the left figure).\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e3. Enterokinase cutting specificity test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/20395-3.jpg?v=1750762129\"\u003e\u003c\/div\u003e\n\u003cp align=\"justify\" class=\"p\" style=\"text-align: center;\"\u003e\u003cstrong\u003eFigure 3. Yeasen enterokinase (20395ES) has less non-specific cutting than Supplier N*\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\" style=\"text-align: left;\"\u003e\u003cstrong\u003e[Note]: \u003c\/strong\u003eUnder the same conditions, Yeasen enterokinase (20395ES) and supplier N's enterokinase were used for enzyme cutting tests. The results show that after purification, the non-specific cutting impurities produced by Yeasen enterokinase (20395ES) are significantly lower than those of Supplier N*.\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e4. Enterokinase repeated freeze-thaw, accelerated stability test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_4d8d90ef-43ee-4b69-85c7-f2b8c4df3c0c.png?v=1750315663\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cstrong\u003eFigure 4. Yeasen enterokinase (Cat#20395ES) repeated freeze-thaw, accelerated stability test shows that enzyme activity did not change significantly.\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003e\u003cstrong\u003e[Note]:\u003c\/strong\u003e Yeasen enterokinase (\u003c\/span\u003e\u003cspan\u003eCat#\u003c\/span\u003e\u003cspan\u003e20395ES) randomly selected 2 batches, repeated freeze-thaw 10 times, 20 times, enzyme activity did not change significantly from the initial enzyme activity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eStorage at 25°C for 7, 16, 32 days, 37°C for 7, 14 days, enzyme activity did not change significantly from the initial enzyme activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eInstructions (Application Example)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e1.  Reaction System:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"17\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"612\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eFusion protein\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"672\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter;\"\u003eμg\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"612\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eEnterokinase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"672\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e0.1-1 U (adjustable according to actua\u003c\/span\u003e\u003cspan\u003el conditions)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"612\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eReaction \u003c\/span\u003e\u003cspan\u003eBuffer \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003e\u003cspan\u003e（\u003c\/span\u003e20 mM Tris-HCl, 50 mM NaCl, 2 \u003c\/span\u003e\u003cspan\u003emM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCaCl\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003epH 8.0\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"672\"\u003e\n\u003cp class=\"15\"\u003e\u003cspan\u003eUp to 50-500 μL(protein co\u003c\/span\u003e\u003cspan\u003encentration 0.1-1 mg\/mL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e2.  Reaction conditio\u003c\/span\u003e\u003cspan\u003ens: Mix well, react at 25°C for 16 hours.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003eNote: Recombinant enterokinase can be diluted with 25 mM Tris-HCl pH 8.0 to a solution \u003c\/span\u003e\u003cspan\u003econtaining 0.1 U per 1  μL\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003efor use.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e1.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eThis enzyme\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ehighly\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003epotent and its activity\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003erelated to the sequence or\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003estructure\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eof the\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eprotein\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eto\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebe\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecut.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eIt\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003erecommended to first d\u003c\/span\u003e\u003cspan\u003eo a gradient of protein input during testing, and to use the most appropriate amount.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e2. The following conditions may have a consistent effect on the activity of rE\u003c\/span\u003e\u003cspan\u003eK:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e1) High ionic strength will inhibit its activity. Under 0.25 M NaCl conditions, \u003c\/span\u003e\u003cspan\u003eit reduces the original activity of rEK by\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eabout 25%; under 2 M NaCl conditions, rEK is almost c\u003c\/span\u003e\u003cspan\u003eompletely inhibited.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e2) Reagents that inhibit its activity may include, \u0026gt;2 M Urea, \u0026gt;20 mM b\u003c\/span\u003e\u003cspan\u003e-ME, \u0026gt;0.1% SDS, \u0026gt;50 mM\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eimidazole, etc.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e3) Conditions of pH\u0026lt;6 or pH\u0026gt;9 will also inhibit its a\u003c\/span\u003e\u003cspan\u003ectivity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e3. Please wear the necessary PPE, suc\u003c\/span\u003e\u003cspan\u003eh lab coat and gloves, to ensure\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eyour health and safety\u003cspan\u003e！\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e4. For research\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003euse only!\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\u003cstrong\u003eRelated reading:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasenbio.com\/blogs\/protein\/recombinant-enterokinase\"\u003e\u003cu\u003e\u003cspan class=\"15\"\u003eUnlocking the Power of Enterokinase\u003c\/span\u003e\u003c\/u\u003e\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 U","offer_id":53294150812013,"sku":"20395ES60","price":60.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294157431149,"sku":"20395ES76","price":255.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20395-1.jpg?v=1778101064","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-bovine-enterokinase-his-expressed-in-yeast-5-u-mu-l-bhz20800026","provider":"BioHippo","version":"1.0","type":"link"}