{"product_id":"recombinant-bovine-interferon-inducible-double-stranded-rna-dependent-protein-kinase-activator-a-prkra-bhp10505589","title":"Recombinant Bovine Interferon-inducible double stranded RNA-dependent protein kinase activator A (PRKRA)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003ePRKRA\u003c\/strong\u003e from Bos taurus (Bovine), produced in E.coli (region 1-313aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 1-313aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His, Myc (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Protein activator of the interferon-induced protein kinase Protein kinase, interferon-inducible double-stranded RNA-dependent activator. Activates EIF2AK2\/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1\/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53\/TP53 association and sumoylation and phosphorylation of p53\/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2\/PKR-dependent manner (By similarity).\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eActivates EIF2AK2\/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1\/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53\/TP53 association and sumoylation and phosphorylation of p53\/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2\/PKR-dependent manner\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving PRKRA\u003c\/li\u003e\n\u003cli\u003eEnzymatic activity measurements with defined substrates\/cofactors (assay-dependent)\u003c\/li\u003e\n\u003cli\u003eCompound screening using concentration–response concepts (assay-dependent)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB Q2HJ92 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q2HJ92 - NCBI Gene search: PRKRA — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PRKRA - Ensembl search: PRKRA — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PRKRA - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053363159405,"sku":"CSB-EP644142BO-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053446390125,"sku":"CSB-EP644142BO-100UG","price":729.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053446422893,"sku":"CSB-EP644142BO-20UG","price":388.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP644142BO-SDS.jpg?v=1772177496","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-bovine-interferon-inducible-double-stranded-rna-dependent-protein-kinase-activator-a-prkra-bhp10505589","provider":"BioHippo","version":"1.0","type":"link"}