{"product_id":"recombinant-caenorhabditis-elegans-transcription-factor-elt-2-elt-2-bhp10516183","title":"Recombinant Caenorhabditis elegans Transcription factor elt-2 (elt-2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Caenorhabditis elegans Transcription factor elt-2 (elt-2) is a recombinant protein preparation derived from Caenorhabditis elegans. It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 1-433aa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e C-terminal 6xHis-tagged; Liquid or Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 54.0 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 85% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eTranscriptional activator that binds to the consensus sequence 5'-[AT]GATA[AG]-3'. Predominantly directs the transcription of intestinal genes such as ges-1, cpr-6, pho-1, ftn-1, vit-2 and lev-11, and itself. Required for gut-specific differentiation, specifically acting with the GATA region-binding transcription factor elt-7 to control normal gene expression and promote normal formation of the intestine. Regulates intestinal gene expression in response to hypoxia to promote longevity. Modulation of longevity may, in part, be the result of regulation of expression of daf-16 isoforms d and f in the intestine. Regulates tissue specific gene expression at basal levels and in response to bacterial infection in the intestine to control innate immunity. Plays a role in the induction of metal-responsive genes, activating gene expression from zinc-activated promoters and iron-dependent promoters and enhancers. May regulate the expression of genes that control sensitivity to oxidative stress, in a mab-3-dependent manner, and osmotic stress, in conjunction with the GATA region-binding transcription factor elt-3. May play a role in sphingolipid signaling by regulating the expression of the sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase. May act with the Notch signaling pathway to promote endodermal gene expression. Has a protective role in response to infection by Gram-negative bacteria such as S.enterica, E.coli, P.aeruginosa and B.pseudomallei, Gram-positive bacterium E.faecalis and fungal pathogen C.neoformans. An association with the 26S proteasome regulatory subunit rpt-6, in part, controls gene expression in response to infection by P.aeruginosa. Regulates gene expression during the recovery phase following a bacterial infection. May act with p38-activated transcription factors to control p38 gene induction in response to bacterial infection. Controls lysosome formation in the intestine by controlling lysosomal gene expression\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eDomain- and isoform-aware assay design to improve biological interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003eQuantitative workflows emphasizing calibration standards, spike-in controls, and cross-lot comparability.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding\/kinetics profiling (SPR\/BLI) to connect biochemical interactions with cellular phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrepare aliquots of elt-2 for reproducible in vitro assays (minimize freeze–thaw).\u003c\/li\u003e\n\u003cli\u003eUse elt-2 as a calibration standard in quantitative assays (standard curve setup).\u003c\/li\u003e\n\u003cli\u003eMeasure binding interactions to elt-2 by SPR\/BLI (kinetic profiling in vitro).\u003c\/li\u003e\n\u003cli\u003eGenerate antibodies to elt-2 and benchmark specificity in ELISA\/WB (control samples).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider species- and isoform-specific differences when comparing results across models or homologs.\u003c\/li\u003e\n\u003cli\u003eFor quantitative assays, include appropriate negative controls and matrix-matched spike-in concepts to assess non-specific signal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (Q10655) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/Q10655 - NCBI Gene search (elt-2) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=elt-2 - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=elt-2 - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059310256493,"sku":"CSB-EP607427CXY-1MG","price":2062.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059464692077,"sku":"CSB-EP607427CXY-100UG","price":480.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059464724845,"sku":"CSB-EP607427CXY-20UG","price":256.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP607427CXY-SDS.jpg?v=1772280381","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-caenorhabditis-elegans-transcription-factor-elt-2-elt-2-bhp10516183","provider":"BioHippo","version":"1.0","type":"link"}