{"product_id":"recombinant-cvb3-vp1-protein-c-his-bhp21414113","title":"Recombinant CVB3 VP1 Protein, C-His","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Viral Protein\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant CVB3 VP1 Protein, C-His\u003c\/strong\u003e is a recombinant \u003cstrong\u003eVP1 capsid protein of coxsackievirus B3 (CVB3)\u003c\/strong\u003e, expressed in \u003cem\u003eE. coli\u003c\/em\u003e as a fragment of the viral genome polyprotein with a C-terminal His tag. The construct spans \u003cstrong\u003eGly571-Phe854\u003c\/strong\u003e of the CVB3 genome polyprotein (UniProt P03313) and has a predicted molecular weight of 32.53 kDa. It is supplied lyophilized at \u0026gt;90% purity as determined by SDS-PAGE.\u003c\/p\u003e\n\u003cp\u003eThe manufacturer lists ELISA, use as an immunogen, SDS-PAGE and Western blot as applications. Bioactivity testing is stated by the manufacturer as in progress, so no functional activity data are provided for this product.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: E. coli · Tag: C-His\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eAbout the Construct\u003c\/h2\u003e\n\u003cp\u003eCVB3 translates its genome as a single polyprotein that is cleaved into structural and non-structural proteins. In the UniProt reference entry P03313, the capsid proteins VP4, VP2, VP3 and VP1 are all derived from the P1 region, and \u003cstrong\u003ecapsid protein VP1 occupies residues 571-851\u003c\/strong\u003e. The expressed range Gly571-Phe854 therefore contains the full VP1 chain and extends three residues (852-854) past its annotated C-terminus, into the start of protease 2A.\u003c\/p\u003e\n\u003cp\u003eThe accession cited by the manufacturer, UniProt P03313, is the genome polyprotein entry for the Nancy strain of CVB3.\u003c\/p\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eVP1 (capsid protein VP1)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eCoxsackievirus B3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eC-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein length\u003c\/th\u003e\n\u003ctd\u003eGly571-Phe854\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAccession\u003c\/th\u003e\n\u003ctd\u003eP03313\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNature\u003c\/th\u003e\n\u003ctd\u003eRecombinant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePredicted molecular weight\u003c\/th\u003e\n\u003ctd\u003e32.53 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;90% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003eNot stated by the manufacturer. Please contact us for this information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage buffer\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. For lot-specific buffer details, see the printed datasheet or the lot-specific COA.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eELISA, Immunogen, SDS-PAGE, Western Blot\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBioactivity\u003c\/th\u003e\n\u003ctd\u003eTesting in progress (manufacturer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eCapsid protein VP1, P1D, Virion protein 1, VP1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003eThe protein is shipped as a lyophilized powder and is dissolved in sterile water before use. Choose the water volume so that the final stock matches the concentration printed on the vial label or datasheet.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eBring the vial and the sterile water to room temperature before you start.\u003c\/li\u003e\n\u003cli\u003eBefore opening, spin the vial down briefly or tap it, so that any powder on the cap or walls collects at the bottom.\u003c\/li\u003e\n\u003cli\u003eAdd water at the volume and stock concentration given on the datasheet. If you need a more concentrated stock than recommended, contact us first.\u003c\/li\u003e\n\u003cli\u003eClose the vial and mix by gentle inversion or on a slow rocker until the liquid has wetted all inner surfaces. Do not vortex and do not pipette the solution up and down.\u003c\/li\u003e\n\u003cli\u003eLet the vial stand at room temperature for 15 minutes or more, agitating gently, before you aliquot or use it.\u003c\/li\u003e\n\u003cli\u003eSplit the stock into single-use aliquots of 10 μL or more in polypropylene or siliconized tubes.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStability and storage\u003c\/th\u003e\n\u003ctd\u003eKeep at 2-8°C when the protein is in frequent use; for longer storage keep it at -20 to -80°C, where it is stable for up to twelve months from receipt. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eProteins normally ship on dry ice or blue ice. Other shipping conditions can be arranged on request.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze-thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for use in diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"50 ug","offer_id":67595255415149,"sku":"VK167012-50UG","price":311.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67595257512301,"sku":"VK167012-100UG","price":411.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":67595257545069,"sku":"VK167012-1MG","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/abinScience_Protein_Placeholder_Image_80ea02a2-4383-4dd3-b73f-b5180cb5d6e6.jpg?v=1790799478","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-cvb3-vp1-protein-c-his-bhp21414113","provider":"BioHippo","version":"1.0","type":"link"}