{"product_id":"recombinant-dictyostelium-discoideum-dna-dependent-protein-kinase-catalytic-subunit-dnapkcs-partial-bhp10505725","title":"Recombinant Dictyostelium discoideum DNA-dependent protein kinase catalytic subunit (dnapkcs), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003ednapkcs\u003c\/strong\u003e from Dictyostelium discoideum (Slime mold), produced in E.coli (region 3910-4250aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 3910-4250aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His, Myc (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as dnapkcs; DDB_G0281167; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; EC 2.7.11.1. Serine\/threonine-protein kinase that acts as a molecular sensor for DNA damage. Is recruited to DNA ends by the Ku70\/Ku80 heterodimer and is involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination (By similarity). This activity is only apparent when DNA damage is administered in G1 phase of the cell cycle. Required for efficient signaling of DNA double-stranded breaks via phosphorylation of H2AX during G1.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eSerine\/threonine-protein kinase that acts as a molecular sensor for DNA damage. Is recruited to DNA ends by the Ku70\/Ku80 heterodimer and is involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. This activity is only apparent when DNA damage is administered in G1 phase of the cell cycle. Required for efficient signaling of DNA double-stranded breaks via phosphorylation of H2AX during G1.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving dnapkcs\u003c\/li\u003e\n\u003cli\u003eEnzymatic activity measurements with defined substrates\/cofactors (assay-dependent)\u003c\/li\u003e\n\u003cli\u003eCompound screening using concentration–response concepts (assay-dependent)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB Q54UC0 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q54UC0 - NCBI Gene search: dnapkcs — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=dnapkcs - Ensembl search: dnapkcs — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=dnapkcs - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053367484781,"sku":"CSB-EP703860DKK-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053456220525,"sku":"CSB-EP703860DKK-100UG","price":729.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053456253293,"sku":"CSB-EP703860DKK-20UG","price":388.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP703860DKK-SDS.jpg?v=1772177509","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-dictyostelium-discoideum-dna-dependent-protein-kinase-catalytic-subunit-dnapkcs-partial-bhp10505725","provider":"BioHippo","version":"1.0","type":"link"}