{"product_id":"recombinant-dnase-i-rnase-free-yeast-5-u-mu-l-bhz20800100","title":"Recombinant DNase I (RNase-free, Yeast, 5 U\/μL)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDNase I, or Deoxyribonuclease I, is an endonuclease that can digest single-stranded or double-stranded DNA. It hydrolyzes phosphodiester bonds to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e and can be activated by divalent metal ions such as Co\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e, Mn\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e, and Zn\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e. In the presence of Mg\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e, DNase I can randomly cut any site of double-stranded DNA; in the presence of Mn\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e2+\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e, DNase I can cut the DNA double strand at the same site, forming a blunt end or a sticky end with 1-2 nucleotides protruding, which can be used for the treatment of various RNA samples.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli align=\"justify\"\u003eRecombinant DNAse I from yeast\u003c\/li\u003e\n\u003cli align=\"justify\"\u003eRNase-free\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003eHigh enzymatic cleavage efficiency.\u003c\/li\u003e\n\u003cli align=\"justify\" class=\"p\"\u003e\n\u003c!-- [if !supportLists]--\u003eMore suitable for applications sensitive to RNase.\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eRemoval of contaminating genomic DNA from RNA samples\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eDegradation of DNA templates in transcription reactions\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eRemoval of gDNA before RNA extraction or reverse transcription.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eRemoval of template DNA in in vitro transcription.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCat.N\u003c\/span\u003e\u003cspan\u003eo\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e14549ES80 \u003c\/span\u003e\u003cspan\u003e\/\u003c\/span\u003e\u003cspan\u003e 14549ES90\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1000 U\u003c\/span\u003e\u003cspan\u003e \/ \u003c\/span\u003e\u003cspan\u003e5000\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eU\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUnit definition:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUsing calf thymus DNA as substrate, at 25℃, pH 5.0, the amount of enzyme required to increase the absorbance of the reaction solution at 260 nm by 0.001 within 1 min is defined as 1 activity unit (Kunitz Unit).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSource\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePichia yeast strain carrying the gene cloned from bovine pancreatic DNase I\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePurity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e≥95%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eReaction conditions\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e37℃, 15-30 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"259\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHeat inactivation conditions\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"542\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAdd 2.5 mM EDTA solution and mix well, then incubate at 65℃ for 10 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cp\u003eGlycerol Content\u003c\/p\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cp\u003eContains Glycerol\u003c\/p\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cstrong\u003eComponents\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cdiv align=\"center\"\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"19.4200%\"\u003e\n\u003cp\u003eComponents No.\u003c\/p\u003e\n\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"53.5200%\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.2400%\"\u003e\n\u003cp\u003e14549ES80\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.7600%\"\u003e\n\u003cp\u003e14549ES90\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"19.4200%\"\u003e\n\u003cp\u003e\u003ca name=\"_Hlk484160428\"\u003e\u003c\/a\u003e14549-A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"53.5200%\"\u003e\n\u003cp\u003eRecombinant DNase I (RNase-free, Yeast) (5 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.2400%\"\u003e\n\u003cp\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.7600%\"\u003e\n\u003cp\u003e1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"19.4200%\"\u003e\n\u003cp\u003e14549-B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"53.5200%\"\u003e\n\u003cp\u003eDNase I Reaction Buffer (10×)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.2400%\"\u003e\n\u003cp\u003e1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.7600%\"\u003e\n\u003cp\u003e5×1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eShipping and Storage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25~-15℃ for two year.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eStrong digestive activity\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14549-3.jpg?v=1747970048\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: center;\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eFigure 1. Plasmid DNA digestion \u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"MsoNormal\" style=\"text-align: left;\"\u003e\u003cspan\u003e1 μg of plasmid DNA was digested using different amounts of DNase I from Y\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eeasen \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eand Supplier A. The results showed that the plasmid DNA removal efficiency of 14549ES was better than that of Supplier A.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c!--[endif]--\u003eNo RNase residue\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\n\u003cimg alt=\"Figure 2 RNase residue verification\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14549-2_1024x1024.png?v=1747906336\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003cspan\u003e \u003c\/span\u003e\n\u003c\/div\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eFigure 2. RNase residue detection\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\" style=\"text-align: left;\"\u003e10 U of Yeasen Recombinant DNase I (RNase-free, Yeast) was incubated with RNA substrate at 37°C for 1 h. Agarose gel electrophoresis analysis showed that no residual RNase activity was detected in the three batches of the enzyme preparation, completely eliminating the risk of RNA sample degradation.\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cstrong\u003e3. RNA extraction application verification\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"Figure 3 RNA extraction application verification\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14549-3_1024x1024.png?v=1747906336\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cstrong\u003e Figure 3. RNA extraction application verification\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\" style=\"text-align: left;\"\u003e20 U of the enzyme preparation was used to treat 12 pre-treatment samples of mouse liver from different sources. RNA extraction and agarose gel electrophoresis analysis showed that the RNA integrity of the enzyme-treated group was good, indicating that this DNase I can effectively digest DNA without affecting the quality of RNA, and fully meets the technical requirements of RNA extraction experiments.\u003c\/p\u003e\n\u003ch3\u003e\u003cbr\u003e\u003c\/h3\u003e\n\n\n\n\n\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"1000 U","offer_id":53294151139693,"sku":"14549ES80","price":55.0,"currency_code":"USD","in_stock":true},{"title":"5000 U","offer_id":53294155563373,"sku":"14549ES90","price":225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/14549-3.jpg?v=1778101063","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-dnase-i-rnase-free-yeast-5-u-mu-l-bhz20800100","provider":"BioHippo","version":"1.0","type":"link"}