{"product_id":"recombinant-drosophila-melanogaster-profilin-chic-bhp10514470","title":"Recombinant Drosophila melanogaster Profilin (chic)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Drosophila melanogaster Profilin (chic) is a recombinant protein preparation derived from Drosophila melanogaster (Fruit fly). It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 1-126aa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e C-terminal 10xHis-tagged; Liquid or Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 23.5 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 85% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it may inhibit the formation of IP3 and DG. This profilin is required for intercellular cytoplasm transport during Drosophila oogenesis . Function in neurons is essential for adult survival, and is important for climbing behavior and activity.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eDomain- and isoform-aware assay design to improve biological interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003eQuantitative workflows emphasizing calibration standards, spike-in controls, and cross-lot comparability.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding\/kinetics profiling (SPR\/BLI) to connect biochemical interactions with cellular phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrepare aliquots of chic for reproducible in vitro assays (minimize freeze–thaw).\u003c\/li\u003e\n\u003cli\u003eUse chic as a calibration standard in quantitative assays (standard curve setup).\u003c\/li\u003e\n\u003cli\u003eMeasure binding interactions to chic by SPR\/BLI (kinetic profiling in vitro).\u003c\/li\u003e\n\u003cli\u003eGenerate antibodies to chic and benchmark specificity in ELISA\/WB (control samples).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider species- and isoform-specific differences when comparing results across models or homologs.\u003c\/li\u003e\n\u003cli\u003eFor quantitative assays, include appropriate negative controls and matrix-matched spike-in concepts to assess non-specific signal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (P25843) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/P25843 - NCBI Gene search (chic) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=chic - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=chic - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059258777965,"sku":"CSB-EP333112DLUd7-1MG","price":1812.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059362193773,"sku":"CSB-EP333112DLUd7-100UG","price":419.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059362226541,"sku":"CSB-EP333112DLUd7-20UG","price":224.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP333112DLUd7-SDS.jpg?v=1772280196","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-drosophila-melanogaster-profilin-chic-bhp10514470","provider":"BioHippo","version":"1.0","type":"link"}