{"product_id":"recombinant-enterokinase-high-concentration-expressed-in-e-coli-bhz20800029","title":"Recombinant Enterokinase, High Concentration, Expressed in E.coli.","description":"\u003cp\u003eRecombinant Enterokinase (rEK) is a highly pure fragment of the recombinant bovine enterokinase light chain. Its amino acid sequence is identical to that of the bovine enterokinase light chain, featuring enzymatic cleavage sites that are as specific as those found in naturally extracted enterokinase, with cutting sites at Asp-Asp-Asp-Asp-Lys. This enables the removal of the fusion protein located at the N-terminus of the protein, eliminating unnecessary fusion tags. Additionally, rEK exhibits higher cleavage activity compared to the natural enzyme.\u003c\/p\u003e\n\u003cp\u003eThe Recombinant Enterokinase is a high-purity, highly active, and highly specific bovine enterokinase produced through recombinant expression in Escherichia coli. Devoid of other proteases, it can effectively cleave fusion proteins over a broad pH range (4.5-9.5) and a wide temperature range. It retains partial activity even in the presence of various detergents and denaturing agents. This product is label-free, and due to its extremely high enzymatic cleavage activity, minimal amounts are needed for cleavage reactions, ensuring no interference with downstream protein applications, making removal unnecessary.\u003c\/p\u003e\n\u003ch3\u003eFeature\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eSpecificity:\u003c\/strong\u003e The enzyme exhibits specificity for a particular amino acid sequence, cleaving at the carboxyl-terminal site containing four aspartic acids followed by lysine (DDDDK).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAnimal free:\u003c\/strong\u003e Produced through recombinant methods, ensuring no contamination from exogenous viruses. The production process does not involve any animal-derived materials.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQuality stability:\u003c\/strong\u003e Batch production guarantees consistently stable batches, with no variations in quality between different batches.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHigh purity:\u003c\/strong\u003e Devoid of other proteases, ensuring non-specific cleavage.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompliance with regulations:\u003c\/strong\u003e Production facilities and environments adhere to relevant regulatory requirements. The manufacturing process adheres to GMP guidelines.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComprehensive quality documentation:\u003c\/strong\u003e Can provide relevant regulatory support documents as per customer requirements.\u003c\/p\u003e\n\u003ch3\u003eSpecification\u003c\/h3\u003e\n\u003ctable style=\"font-weight: 400;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003eExpressed in \u003cem\u003eE.coli.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55px;\"\u003e\n\u003ctd style=\"height: 55px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eMolecular Weight\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 55px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e25.85 kDa\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eAppearance\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cspan\u003eClear, colorless liquid.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003c\/b\u003e\u003cb\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e≥5 U\/uL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 94px;\"\u003e\n\u003ctd style=\"height: 94px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eActivity Definition\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 94px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cspan\u003eOne unit of activity is defined as the amount of enzyme required to cleave 95% of\u003cstrong\u003e 500 μg\u003c\/strong\u003e of a 30KD fusion protein containing enterokinase cleavage sites, under the following conditions: 25°C, 12-16 hours, in a buffer system consisting of 25 mM Tris-HCl, pH 8.0.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e＜1 EU\/μg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"height: 35px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eHCP\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 35px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e≤0.01%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 30.7396px;\"\u003e\n\u003ctd style=\"height: 30.7396px; width: 35.9356%;\"\u003e\u003cb\u003eTag\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"height: 30.7396px; width: 61.2969%;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55px;\"\u003e\n\u003ctd style=\"height: 55px; width: 35.9356%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003cb\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 55px; width: 61.2969%;\"\u003e\n\u003cp style=\"font-weight: 400;\"\u003e50 mM Tris-HCl，pH8.0，250 mM NaCl，2 mM CaCl2，50% Glycerol\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003e\u003cspan\u003eThe amount of recombinant protein cleaved per unit of Enterokinase (EK) \u003c\/span\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center;\"\u003e\n\u003cp\u003eSuppliers\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.0171%; text-align: center;\"\u003e\n\u003cp\u003e Yeasen 5 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 34.9829%; text-align: center;\"\u003e\n\u003cp\u003e Thermo 1 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26%; text-align: center;\"\u003e\n\u003cp\u003e NEB 16 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15%; text-align: center;\"\u003e\n\u003cp\u003eActivity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 32.0171%; text-align: center;\"\u003e\n\u003cp\u003e500 μg\/U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 34.9829%; text-align: center;\"\u003e\n\u003cp\u003e20 μg\/U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 26%; text-align: center;\"\u003e\n\u003cp\u003e25 μg\/U\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cbr\u003e\u003c\/h3\u003e\n\u003ch3\u003eStorage \u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eStore at -25 to -15°C for 1 years. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"100 U","offer_id":53294148911469,"sku":"20401ES60","price":60.0,"currency_code":"USD","in_stock":true},{"title":"500 U","offer_id":53294155202925,"sku":"20401ES76","price":255.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/20401.webp?v=1778113412","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-enterokinase-high-concentration-expressed-in-e-coli-bhz20800029","provider":"BioHippo","version":"1.0","type":"link"}