{"product_id":"recombinant-epstein-barr-virus-latent-membrane-protein-1-lmp1-partial-bhp10516113","title":"Recombinant Epstein-Barr virus Latent membrane protein 1 (LMP1), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Epstein-Barr virus Latent membrane protein 1 (LMP1), partial is a recombinant protein preparation derived from Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4). It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 185-386aa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e N-terminal 6xHis-taggedand C-terminal Myc-tagged; Liquid or Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 28.2 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 85% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eActs as a CD40 functional homolog to prevent apoptosis of infected B-lymphocytes and drive their proliferation. Functions as a constitutively active tumor necrosis factor receptor that induces the activation of several signaling pathways, including those of the NF-kappa-B family. LMP1 signaling leads to up-regulation of antiapoptotic proteins and provide growth signals in latently infected cells. Interacts with host UBE2I and subsequently affects the sumoylation state of several cellular proteins. For example, induces the sumoylation of host IRF7 thereby limiting its transcriptional activity and modulating the activation of innate immune responses. Also inhibits host IFN-alpha-stimulated STAT2 nuclear translocation and interferon-stimulated response element transcriptional activity by interacting with and inhibiting host TYK2. Induces SUMO expression during viral latency thereby dysregulating the host sumoylation processes\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAntigen design and domain selection to better capture neutralizing versus non-neutralizing antibody responses.\u003c\/li\u003e\n\u003cli\u003eHigh-throughput serology and antibody screening using standardized antigens and plate-based formats.\u003c\/li\u003e\n\u003cli\u003eIntegrating binding kinetics with epitope mapping to support variant-aware immunology studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCoat plates with LMP1 antigen for ELISA antibody titration (serum\/plasma).\u003c\/li\u003e\n\u003cli\u003eScreen anti-LMP1 antibodies by indirect ELISA and immunoblot readouts.\u003c\/li\u003e\n\u003cli\u003eMap antigenic epitopes using LMP1 fragments\/domains (in vitro binding assays).\u003c\/li\u003e\n\u003cli\u003eDevelop antigen-capture assays using LMP1 as a standard (spike-in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAntigenic proteins may contain immunodominant regions; domain choice can affect assay readouts and cross-reactivity.\u003c\/li\u003e\n\u003cli\u003eInclude relevant negative controls (e.g., unrelated antigens) and dilution series to support interpretation of binding signals.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (P03230) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/P03230 - NCBI Gene search (LMP1) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LMP1 - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LMP1 - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059307602285,"sku":"CSB-EP355987EFA1-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059459219821,"sku":"CSB-EP355987EFA1-100UG","price":578.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059459252589,"sku":"CSB-EP355987EFA1-20UG","price":306.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP355987EFA1-SDS.jpg?v=1772280370","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-epstein-barr-virus-latent-membrane-protein-1-lmp1-partial-bhp10516113","provider":"BioHippo","version":"1.0","type":"link"}