{"product_id":"recombinant-escherichia-coli-ribonuclease-e-rne-partial-bhp10514187","title":"Recombinant Escherichia coli Ribonuclease E (rne), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Escherichia coli Ribonuclease E (rne), partial is a recombinant protein preparation derived from Escherichia coli (strain K12). It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 35-125aa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Yeast (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e C-terminal 6xHis-Myc-tagged; Liquid or Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 13.9 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eEndoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs. Can also process other RNA species, such as RNAI, a molecule that controls the replication of ColE1 plasmid, and the cell division inhibitor DicF-RNA. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic process. Required to initiate rRNA degradation during both starvation and quality control; acts after RNase PH (rph) exonucleolytically digests the 3'-end of the 16S rRNA . Degradation of 16S rRNA leads to 23S rRNA degradation . Processes the 3 tRNA(Pro) precursors immediately after the 3'-CCA to generate the mature ends . Prefers 5'-monophosphorylated substrates over 5'-triphosphorylated substrates . 5'-monophosphate-assisted cleavage requires at least 2 and preferably 3 or more unpaired 5'-terminal nucleotides. The optimal spacing between the 5' end and the scissile phosphate appears to be 8 nucleotides. Any sequence of unpaired nucleotides at the 5'-end is tolerated .\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eDomain- and isoform-aware assay design to improve biological interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003eQuantitative workflows emphasizing calibration standards, spike-in controls, and cross-lot comparability.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding\/kinetics profiling (SPR\/BLI) to connect biochemical interactions with cellular phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrepare aliquots of rne for reproducible in vitro assays (minimize freeze–thaw).\u003c\/li\u003e\n\u003cli\u003eUse rne as a calibration standard in quantitative assays (standard curve setup).\u003c\/li\u003e\n\u003cli\u003eMeasure binding interactions to rne by SPR\/BLI (kinetic profiling in vitro).\u003c\/li\u003e\n\u003cli\u003eGenerate antibodies to rne and benchmark specificity in ELISA\/WB (control samples).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider species- and isoform-specific differences when comparing results across models or homologs.\u003c\/li\u003e\n\u003cli\u003eFor quantitative assays, include appropriate negative controls and matrix-matched spike-in concepts to assess non-specific signal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (P21513) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/P21513 - NCBI Gene search (rne) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=rne - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=rne - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059250913645,"sku":"CSB-YP324207ENV-1MG","price":2959.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059346465133,"sku":"CSB-YP324207ENV-100UG","price":826.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059346497901,"sku":"CSB-YP324207ENV-20UG","price":436.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-YP324207ENV-SDS.jpg?v=1772280105","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-escherichia-coli-ribonuclease-e-rne-partial-bhp10514187","provider":"BioHippo","version":"1.0","type":"link"}