{"product_id":"recombinant-hcd275-muig-bhp14700035","title":"Recombinant hCD275-muIg","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eThe inducible costimulator CD278 (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process, each secondary signal resulting in a discrete cytokine secretion profile displayed by the activated T cell.1 Both processes are effectively down regulated by CD152 (CTLA-4) engagement.2 Human CD275(ICOSL, GL50, B7RP-1, B7-H2) is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for CD278(ICOS).3 It has recently been shown that at least two RNA splice variants exist for this molecule in mouse and human, possibly serving to regulate this costimulatory process. 4\u003c\/p\u003e\n\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eCHO Cells\u003c\/span\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant hCD275-muIg\u003c\/strong\u003e is a recombinant human fusion protein produced in \u003cstrong\u003eCHO Cells\u003c\/strong\u003e cells. Molecular Structure: A soluble dimeric fusion protein consisting of a portion of the extracellular domain of human CD275 (GL50, ICOSL, B7-H2, B7RP-1) (213aa): dtqekevramvgsdvelscacpegsrfdlndvyvywqtsesktvvtyhipqnsslenvdsryrnralmspagmlrgdfslrlfnvtpqdeqkfhclvlsqslgfqevlsvevtlhvaanfsvpvvsaphspsqdeltftctsingyprpnvywinktdnslldqalqndtvflnmrglydvvsvlriartpsvnigccienvllqqnltvgsq with linking amino acids: gt\u003c\/p\u003e\n\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n  \u003ctr\u003e\n\u003cth\u003eFusion Format\u003c\/th\u003e\n\u003ctd\u003eMurine IgG Fc Fusion (muIg)\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eExpression System\u003c\/th\u003e\n\u003ctd\u003eCHO Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003e50.4 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003e50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 2 - 5°C. Freeze\/Thawing is not recommended.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eValidated Applications\u003c\/th\u003e\n\u003ctd\u003eELISA, Flow Cytometry\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eAvailable Conjugates\u003c\/th\u003e\n\u003ctd\u003eBiotin, Unconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003e✔ Research Use Only (RUO)\u003c\/span\u003e\u003c\/p\u003e\n\n\u003ch2\u003eFunctional Activity\u003c\/h2\u003e\n\u003cp\u003eCD275-muIg binds with high affinity to cell surface CD278 (ICOS) expressed on CD8 positive human T cells. It’s binding is blocked by pre incubation with recombinant 278-muIg (cat.no. 517-020)\u003c\/p\u003e\n\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e Not intended for diagnostic, therapeutic, or clinical use. Handle according to good laboratory practice (GLP). Consult the Safety Data Sheet (SDS) prior to use.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eBrowse our full collection of \u003ca href=\"\/collections\/proteins\"\u003erecombinant proteins\u003c\/a\u003e and \u003ca href=\"\/collections\/antibodies\"\u003eantibodies\u003c\/a\u003e for immunology and immune checkpoint research.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Ancell Corporation","offers":[{"title":"Purified (with Antibiotic) \/ 25 ug","offer_id":53339389297005,"sku":"575-020","price":420.0,"currency_code":"USD","in_stock":true},{"title":"Purified (Preservative-free) \/ 25 ug","offer_id":53339391656301,"sku":"575-820","price":420.0,"currency_code":"USD","in_stock":true},{"title":"Biotin conjugate \/ 25 ug","offer_id":53339391689069,"sku":"575-030","price":510.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/recombinant-hcd275-muig-bhp14700035","provider":"BioHippo","version":"1.0","type":"link"}