{"product_id":"recombinant-heteroscodra-maculata-delta-theraphotoxin-hm1a-bhp10504971","title":"Recombinant Heteroscodra maculata Delta-theraphotoxin-Hm1a","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Heteroscodra maculata Delta-theraphotoxin-Hm1a is a recombinant protein reagent for research-use applications such as assay development, binding studies, and mechanistic experiments. It corresponds to \u003cstrong\u003eDelta-theraphotoxin-Hm1a\u003c\/strong\u003e (Heteroscodra maculata (Togo starburst tarantula) (Togo starburst baboon spider)) and is intended for RUO workflows where a defined protein standard or functional input is needed.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Yeast (expression context can influence folding and PTMs).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression region:\u003c\/strong\u003e 1-35aa (region choice can affect activity and binding readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate(s)\/tag:\u003c\/strong\u003e Tag-Free (can support detection or purification depending on format).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 4 kDa (useful for interpreting gel migration and size-exclusion profiles).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eWhen comparing results across assays, consider that expression system and expressed region can alter glycosylation, disulfide formation, and oligomerization state, which may shift apparent potency or binding behavior in vitro.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eGating modifier toxin that principally inhibits inactivation of the mammalian voltage-gated sodium channel Nav1.1\/SCN1A. When tested against human Nav1.1\/SCN1A-Nav1.8\/SCN10A alpha subunits heterologously expressed in Xenopus oocytes, the toxin inhibits inactivation of Nav1.1 (EC(50)=38 nM), with substantially weaker effects on Nav1.2\/SCN2A (EC(50)=236 nM) and Nav1.3\/SCN3A (EC(50)=220 nM). The toxin inhibits several subtypes of voltage-gated potassium channels with significantly lower potency than its effects on Nav1.1\/SCN1A. It is a moderately potent blocker of Kv2.1\/KCNB1, Kv2.2\/KCNB2, Kv4.1\/KCND1, Kv4.2\/KCND2 and Kv4.3\/KCND3 and has little effect on Kv1.1\/KCNA1, Kv1.2\/KCNA2, Kv1.3\/KCNA3, Kv1.4\/KCNA4, Kv1.5\/KCNA5, Kv1.6\/KCNA6 and Kv3.4\/KCNC4. The binding affinity and subtype selectivity of the toxin is determined by residues within both the S1-S2 and S3-S4 loops of the domain IV voltage sensor. Intracerebroventricular injection into mice elicits convulsions, spasms, tremors and rapid death. When injected into mouse hindpaw, the toxin elicits an immediate and robust response to pain. Intraplantar injection of toxin does not cause neurogenic inflammation or alter sensitivity to heat, indicative of a modality-specific effect on mechanosensitive neurons\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eReagent standardization: using recombinant proteins as reference materials for quantitative calibration and cross-study comparability.\u003c\/li\u003e\n\u003cli\u003eInteraction-focused studies: mapping binding partners, affinity changes, and structure–function relationships across variants or domains.\u003c\/li\u003e\n\u003cli\u003eMulti-omic readouts: combining recombinant perturbations with transcript, protein, and functional endpoints to connect mechanism to phenotype.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAssay development and validation: use as a defined input or standard where protein identity is required.\u003c\/li\u003e\n\u003cli\u003eBinding studies: evaluate interaction strength and specificity using plate-based or biophysical formats.\u003c\/li\u003e\n\u003cli\u003eCell-response profiling: add protein to cultured cells and interpret downstream marker changes with appropriate controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpretation is most robust when signal changes are evaluated relative to matched controls (buffer-only, unrelated protein controls, or pathway controls) and when readouts are compared across dose and time.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eIsoforms and PTMs can influence binding and activity; ensure the expressed region and expression system match your experimental needs.\u003c\/li\u003e\n\u003cli\u003eSpecies differences may affect receptor binding or antibody recognition; confirm species\/source alignment with your model.\u003c\/li\u003e\n\u003cli\u003eUse concept-level controls such as negative controls (no protein), matrix controls, or orthogonal readouts to support conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt keyword search: https:\/\/www.uniprot.org\/uniprotkb?query=Delta-theraphotoxin-Hm1a - NCBI Gene search: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Delta-theraphotoxin-Hm1a - PubMed search: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Delta-theraphotoxin-Hm1a - Ensembl search: https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Delta-theraphotoxin-Hm1a - Reactome Pathway Browser: https:\/\/reactome.org\/content\/query?q=Delta-theraphotoxin-Hm1a --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053073064301,"sku":"CSB-YP351577HGU-1MG","price":3125.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053247160685,"sku":"CSB-YP351577HGU-100UG","price":992.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053247193453,"sku":"CSB-YP351577HGU-20UG","price":602.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-YP351577HGU-SDS.jpg?v=1772172982","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-heteroscodra-maculata-delta-theraphotoxin-hm1a-bhp10504971","provider":"BioHippo","version":"1.0","type":"link"}