{"product_id":"recombinant-histone-h3-antibody-bha17103714","title":"Recombinant Histone H3 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eRecombinant Histone H3 antibody supplied as a purified reagent for WB, ELISA, ICC in All Species samples. This product is a recombinant rabbit monoclonal antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Recombinant Rabbit Monoclonal; host Rabbit; isotype Rabbit IgG; clone RM186.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein A purified from animal origin-free supernatant.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e All Species.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB, ELISA, ICC.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e An unmodified peptide corresponding to the N-terminus of Histone H3 was used as the immunogen for this recombinant Histone H3 antibody..\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eRecombinant Histone H3 is the intended antigen for this primary antibody. Reported biological context includes: This recombinant Histone H3 antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The recombinant Histone H3 antibody binding specificity allows for modifications of Arg2, Thr3, and\/or other modifications in Histone H3.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003ePerturbation and chemical biology: acetylation\/deacetylation pathways are frequently interrogated with inhibitors and genetic perturbations to separate direct regulation from adaptive responses.\u003c\/li\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e   \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e   \u003cli\u003eELISA: support quantitative detection workflows and assay development where a defined antibody reagent is needed for capture or detection.\u003c\/li\u003e   \u003cli\u003eImmunocytochemistry (ICC): visualize intracellular distribution and morphology-linked changes in cultured cells.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProtKB entry (Q16695) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/Q16695\/entry - NCBI Gene search (Recombinant Histone H3) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Recombinant+Histone+H3 - Ensembl search (Recombinant Histone H3) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Recombinant+Histone+H3 - PubMed search (Recombinant Histone H3) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Recombinant+Histone+H3 - Reactome pathway search (Recombinant Histone H3) — Reactome — https:\/\/reactome.org\/content\/query?q=Recombinant+Histone+H3 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"1 mg\/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 100 ug","offer_id":53043246825837,"sku":"R20228-100UG","price":485.0,"currency_code":"USD","in_stock":true},{"title":"1 mg\/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 25 ug","offer_id":53043678151021,"sku":"R20228-25UG","price":259.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_706bb813-5e85-4b93-95d0-6f466b53a3d6.jpg?v=1771934400","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-histone-h3-antibody-bha17103714","provider":"BioHippo","version":"1.0","type":"link"}