{"product_id":"recombinant-human-activin-a-protein-bhp22200001","title":"Recombinant Human Activin A Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · TGF-β Superfamily\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human Activin A Protein\u003c\/strong\u003e is a tag-free, disulfide-linked homodimer of the inhibin beta A subunit (gene \u003cem\u003eINHBA\u003c\/em\u003e), expressed in \u003cstrong\u003eHEK293 human cells\u003c\/strong\u003e. It is manufactured in an animal-free, xeno-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eActivin A is commonly used in human iPSC and ESC differentiation protocols, for primitive-streak and mesendoderm induction, definitive endoderm specification and the pancreatic and hepatic programs that follow. Expression in human cells supports mammalian folding and post-translational processing of the dimeric ligand. Each lot is tested for purity, endotoxin and biological activity before release, and the product has been reported in peer-reviewed studies on chemical reprogramming, pluripotent stem-cell culture and directed differentiation.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: HEK293 human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eActivin A belongs to the TGF-β superfamily. It signals through heteromeric receptor complexes made of type II Activin receptors (mainly ACVR2A and ACVR2B) and the type I receptor ALK4 (ACVR1B). Receptor activation leads to phosphorylation of SMAD2 and SMAD3, which associate with SMAD4 and regulate context-dependent transcription in the nucleus.\u003c\/p\u003e\n\u003cp\u003eActivin signaling takes part in embryonic patterning, pluripotency, germ-layer specification, tissue morphogenesis, reproductive biology, inflammation, fibrosis, hematopoiesis and tissue repair. In pluripotent stem-cell work, the lineage that results is shaped by how much Activin A is added and for how long, by the starting cell state and medium, and by crosstalk with WNT, BMP, FGF and PI3K\/AKT signaling, so dose and timing need to be optimized for each cell line and target lineage.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eActivin A is mainly used in the early stages of directed differentiation of human pluripotent stem cells. The table summarizes where it is typically placed in a workflow; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of Activin A\u003c\/th\u003e\n\u003cth\u003eTypical stage\u003c\/th\u003e\n\u003cth\u003eOther signals often used\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDefinitive endoderm\u003c\/td\u003e\n\u003ctd\u003eDrives early endodermal programs\u003c\/td\u003e\n\u003ctd\u003eEarly differentiation, after pluripotent-cell preparation\u003c\/td\u003e\n\u003ctd\u003eWNT modulation; FGF\/PI3K control depending on the protocol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMesendoderm \/ primitive streak\u003c\/td\u003e\n\u003ctd\u003eSupports early germ-layer and primitive-streak-like transitions\u003c\/td\u003e\n\u003ctd\u003eVery early differentiation\u003c\/td\u003e\n\u003ctd\u003eWNT activation; BMP or FGF depending on the target lineage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePancreatic differentiation\u003c\/td\u003e\n\u003ctd\u003eEstablishes definitive endoderm\u003c\/td\u003e\n\u003ctd\u003eUpstream of pancreatic progenitor specification\u003c\/td\u003e\n\u003ctd\u003eRetinoid signaling, FGF, BMP modulation and pathway-specific small molecules\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHepatic differentiation\u003c\/td\u003e\n\u003ctd\u003eSupports early definitive-endoderm formation\u003c\/td\u003e\n\u003ctd\u003eBefore hepatic specification and maturation\u003c\/td\u003e\n\u003ctd\u003eBMP, FGF, HGF, OSM and maturation supplements\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganoid research\u003c\/td\u003e\n\u003ctd\u003eSupports selected early developmental stages\u003c\/td\u003e\n\u003ctd\u003eDepends on organoid type and stage\u003c\/td\u003e\n\u003ctd\u003eWNT, BMP, FGF, EGF and tissue-specific signals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChemical reprogramming\u003c\/td\u003e\n\u003ctd\u003eSupports selected pluripotent-state or reprogramming conditions\u003c\/td\u003e\n\u003ctd\u003eProtocol-specific\u003c\/td\u003e\n\u003ctd\u003eDefined small-molecule combinations and other growth factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePluripotent stem-cell culture\u003c\/td\u003e\n\u003ctd\u003eActivin\/Nodal pathway support in some defined culture systems\u003c\/td\u003e\n\u003ctd\u003ePlatform-dependent; the requirement varies between systems\u003c\/td\u003e\n\u003ctd\u003eDefined basal medium and matrix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the stage Activin A is used for (pluripotency support, mesendoderm, definitive endoderm or a later step) and note the basal medium, WNT\/BMP\/FGF conditions and small molecules used with it.\u003c\/li\u003e\n\u003cli\u003eOptimize concentration and exposure time for each cell line. Published concentrations vary widely between applications, and exposure beyond the intended window can shift the resulting cell population.\u003c\/li\u003e\n\u003cli\u003eKey variables include starting-cell quality, seeding density at induction, medium and supplements, matrix or coating, and WNT, FGF, BMP and PI3K\/AKT activity.\u003c\/li\u003e\n\u003cli\u003eFor endoderm work, confirm the result with morphology and markers such as SOX17 and FOXA2, and check that the cells can progress to the intended downstream lineage.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eActivin A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eINHBA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHEK293 human cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eDisulfide-linked homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eTGF-β superfamily\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGene symbol\u003c\/th\u003e\n\u003ctd\u003eINHBA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt\u003c\/th\u003e\n\u003ctd\u003eP08476\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free manufacturing format\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 25 kDa. Refer to the lot-specific Certificate of Analysis (CoA) and SDS-PAGE profile for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized, carrier-protein free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInhibition of murine MPC-11 plasmacytoma cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEC50\u003c\/th\u003e\n\u003ctd\u003e6.68–10.02 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Stem Cell Culture, 3D cell culture, Cell Reprogramming, Immunotherapy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by inhibition of murine MPC-11 plasmacytoma cell proliferation. The typical EC50 is 6.68–10.02 ng\/mL. Each lot is tested for purity, endotoxin and biological activity before release.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and avoid aggressive vortexing.\u003c\/li\u003e\n\u003cli\u003ePrepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641413534061,"sku":"HST-A-0010","price":175.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641425265005,"sku":"HST-A-0100","price":1219.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/activina-500x500.webp?v=1791572710","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-activin-a-protein-bhp22200001","provider":"BioHippo","version":"1.0","type":"link"}