{"product_id":"recombinant-human-atg4b-protein-n-his-bhp21400024","title":"Recombinant Human ATG4B Protein, N-His","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eWhat is ATG4B?\u003c\/strong\u003e Cysteine protease ATG4B (Autophagy-related cysteine endopeptidase 1) (ATG4B) is a Cysteine protease that processes ATG8-family proteins (LC3\/GABARAP) in autophagy systems. This target is supplied here as a \u003cstrong\u003erecombinant protein reagent for research use only\u003c\/strong\u003e, enabling controlled biochemical and cell-free interrogation of binding, activity, and interaction logic with a traceable input material.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSubcellular localization (research context):\u003c\/strong\u003e Cytosolic; functionally associated with autophagy machinery and membrane-associated ATG8 conjugation cycles.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDomain \/ architecture (why fragments matter):\u003c\/strong\u003e A papain-like cysteine protease fold adapted for ATG8-family substrate recognition.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat this recombinant protein represents:\u003c\/strong\u003e This product is a \u003cstrong\u003erecombinant Human ATG4B\u003c\/strong\u003e protein  corresponding to \u003cstrong\u003eMet1-Leu393\u003c\/strong\u003e with a \u003cstrong\u003eN-His\u003c\/strong\u003e tag. It is expressed in \u003cstrong\u003eE. coli\u003c\/strong\u003e and supplied as a purified RUO reagent.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\n\u003cp\u003eATG4B is central to autophagy mechanism studies because it catalyzes priming and delipidation steps that control ATG8-family cycling. Recombinant ATG4B enables defined protease assays, substrate specificity mapping, and inhibitor benchmarking.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eMolecular characteristics relevant to experimental design\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein class:\u003c\/strong\u003e Cysteine protease that processes ATG8-family proteins (LC3\/GABARAP) in autophagy systems\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion \/ construct:\u003c\/strong\u003e Met1-Leu393\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApprox. molecular weight:\u003c\/strong\u003e 46.46 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;90%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e Affinity-chromatography\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003ePost-translational and conformational considerations:\u003c\/strong\u003e Prokaryotic expression typically yields a non-glycosylated recombinant form. This is often appropriate for cytosolic enzymes and many binding studies, while secreted or multi-domain extracellular targets can behave differently when native PTMs are required.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eExpression and purification context (quality transparency for RUO)\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E. coli\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion expressed:\u003c\/strong\u003e Met1-Leu393\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag:\u003c\/strong\u003e N-His\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification:\u003c\/strong\u003e Affinity-chromatography\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;90%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation (context):\u003c\/strong\u003e Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEndotoxin:\u003c\/strong\u003e Not provided in the public listing (contact for details if your assay is endotoxin-sensitive).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003ch2\u003eResearch interpretation\u003c\/h2\u003e\n\u003cp\u003eATG4B recombinant reagents are most valuable when you need a \u003cstrong\u003edefined molecular input\u003c\/strong\u003e to reduce variability from endogenous expression and processing.\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 ug","offer_id":53000606974317,"sku":"HB598012-100UG","price":311.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53000607007085,"sku":"HB598012-1MG","price":1627.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HB598012-SDSPAGE-1.jpg?v=1770274658","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-atg4b-protein-n-his-bhp21400024","provider":"BioHippo","version":"1.0","type":"link"}