{"product_id":"recombinant-human-atp-binding-cassette-sub-family-b-member-8-mitochondrial-abcb8-partial-bhp10503925","title":"Recombinant Human ATP-binding cassette sub-family B member 8, mitochondrial (ABCB8), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Homo sapiens (Human): Human ATP-binding cassette sub-family B member 8, mitochondrial (ABCB8), partial corresponding to amino acids 38–693. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 38–693 (656 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 3TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Homo sapiens (Human). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt Q9NUT2. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eHuman ATP-binding cassette sub-family B member 8, mitochondrial (ABCB8), partial belongs to the ATP-binding cassette (ABC) transporter superfamily. ABC transporters use ATP binding and hydrolysis to power conformational changes that move substrates across membranes or regulate transport-related processes. In eukaryotes, mitochondrial ABC transporters are commonly studied in the context of organelle homeostasis, including metabolite transport and redox\/metal handling. For membrane transporters, the relationship between sequence, transmembrane architecture, and substrate specificity is a central theme. Also reported as: Mitochondrial ATP-binding cassette 1; ; M-ABC1.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMechanistic transporter studies that link ATPase activity, conformational cycling, and substrate recognition (often integrating structural and biochemical data).\u003c\/li\u003e\n\u003cli\u003eDisease- and physiology-oriented research connecting ABC transporters to cellular homeostasis and stress responses (interpreted in model systems).\u003c\/li\u003e\n\u003cli\u003eDevelopment of inhibitors\/modulators and assays that probe transporter function, specificity, and regulation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAntigen production for antibody development or binder screening against defined regions of an ABC transporter.\u003c\/li\u003e\n\u003cli\u003eDomain-focused biochemical studies (e.g., interaction mapping with partner proteins or lipids) where the expressed region is critical for interpretation.\u003c\/li\u003e\n\u003cli\u003eComparative studies across orthologs or variants to explore conserved motifs and potential functional differences.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Human ATP-binding cassette sub-family B member 8, mitochondrial (ABCB8), partial (Q9NUT2) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q9NUT2\/entry\n- NCBI Gene search: ABCB8 Homo sapiens — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ABCB8%20Homo%20sapiens\n- PubMed search: ABCB8 review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ABCB8%20review\n- InterPro search: ABCB8 — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/ABCB8\/\n- Ensembl Gene Summary: ABCB8 (Homo_sapiens) — Ensembl — https:\/\/www.ensembl.org\/Homo_sapiens\/Gene\/Summary?g=ABCB8\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207322394989,"sku":"CSB-CF868325HU-100UG","price":1462.6,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320591311213,"sku":"CSB-CF868325HU-20UG","price":904.34,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF868325HU-SDS.jpg?v=1778623124","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-atp-binding-cassette-sub-family-b-member-8-mitochondrial-abcb8-partial-bhp10503925","provider":"BioHippo","version":"1.0","type":"link"}