{"product_id":"recombinant-human-bmp-4-protein-bhp22200003","title":"Recombinant Human BMP-4 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · TGF-β Superfamily\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human BMP-4 Protein\u003c\/strong\u003e is a tag-free, disulfide-linked homodimer of bone morphogenetic protein 4, expressed in \u003cstrong\u003eHEK293 human cells\u003c\/strong\u003e. It is made in an animal-free, xeno-free format and supplied lyophilized without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eBMP-4 is a standard tool for steering early lineage decisions in human iPSC and ESC differentiation: primitive-streak and mesoderm induction, followed by cardiovascular, endothelial, hemogenic-endothelium and hematopoietic programs. It is also used in organoid and developmental-biology models and in bone, cartilage and tissue-engineering research. Production in human cells supports mammalian folding and processing of the dimer, every lot is checked for purity, endotoxin and biological activity, and the protein has been reported in peer-reviewed work on chemical reprogramming, pluripotent stem-cell differentiation and hematopoietic development.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: HEK293 human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eBone Morphogenetic Protein 4 is a secreted member of the transforming growth factor beta (TGF-β) superfamily. BMPs were first described through their effects on bone formation, but BMP-4 acts much more widely, in embryonic patterning, germ-layer specification, tissue morphogenesis and cell-fate control.\u003c\/p\u003e\n\u003cp\u003eBMP-4 binds heteromeric complexes of type I and type II BMP receptors. Receptor activation phosphorylates the receptor-regulated SMAD1, SMAD5 and SMAD9, which pair with SMAD4 and move into the nucleus to control context-dependent gene expression. BMP-4 signaling can also engage non-SMAD pathways and cross-talk with WNT, Activin\/Nodal, FGF and VEGF networks.\u003c\/p\u003e\n\u003cp\u003eIn pluripotent stem-cell systems BMP-4 is usually added early to promote primitive-streak-like and mesodermal states; later pathway modulation then directs these cells toward cardiovascular, hematopoietic, endothelial, skeletal, hepatic or other fates. Because the outcome depends on concentration, timing, medium, cell state and companion signals, BMP-4 is best treated as a context-dependent developmental cue, with dose and exposure optimized for each cell line, basal medium and target lineage.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eBMP-4 is mainly used at the start of directed differentiation, where it sets up primitive-streak-like and mesodermal states that later signals turn into specific lineages. The table summarizes typical placements and the factors the manufacturer lists alongside BMP-4; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of BMP-4\u003c\/th\u003e\n\u003cth\u003eRepresentative factors\u003c\/th\u003e\n\u003cth\u003eExample outputs\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrimitive-streak induction\u003c\/td\u003e\n\u003ctd\u003eStarts primitive-streak-like programs in the first differentiation stage\u003c\/td\u003e\n\u003ctd\u003eBMP-4 + Activin A + CHIR99021\u003c\/td\u003e\n\u003ctd\u003eMesoderm-competent intermediates from iPSCs\/ESCs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMesoderm specification\u003c\/td\u003e\n\u003ctd\u003eBuilds the mesoderm foundation for downstream lineages\u003c\/td\u003e\n\u003ctd\u003eBMP-4 + VEGF + FGF2\u003c\/td\u003e\n\u003ctd\u003eCardiovascular, hematopoietic, MSC and skeletal precursors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCardiovascular differentiation\u003c\/td\u003e\n\u003ctd\u003eCardiac mesoderm induction and endothelial specification\u003c\/td\u003e\n\u003ctd\u003eVEGF165, FGF2, WNT modulation\u003c\/td\u003e\n\u003ctd\u003eCardiomyocytes, endothelial cells, vascular smooth muscle cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHemogenic endothelium\u003c\/td\u003e\n\u003ctd\u003eSupports mesoderm and hemogenic endothelium before endothelial-to-hematopoietic transition\u003c\/td\u003e\n\u003ctd\u003eBMP-4 + VEGF + SCF + FLT3 Ligand\u003c\/td\u003e\n\u003ctd\u003eHemogenic endothelial populations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHSPC generation\u003c\/td\u003e\n\u003ctd\u003eEarly hematopoietic specification\u003c\/td\u003e\n\u003ctd\u003eBMP-4 + SCF + TPO + FLT3 Ligand\u003c\/td\u003e\n\u003ctd\u003eHematopoietic progenitors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBone and cartilage\u003c\/td\u003e\n\u003ctd\u003eRegulates osteogenesis and chondrogenesis\u003c\/td\u003e\n\u003ctd\u003eBMP signaling, TGF-β family\u003c\/td\u003e\n\u003ctd\u003eMSC osteogenic and chondrogenic differentiation, bone and cartilage repair models\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganoids and developmental models\u003c\/td\u003e\n\u003ctd\u003eEmbryonic patterning signal\u003c\/td\u003e\n\u003ctd\u003eStage-specific BMP, WNT, Activin and FGF\u003c\/td\u003e\n\u003ctd\u003eGastruloids, embryoid bodies, kidney, liver and heart organoids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTreat BMP-4 as a context-dependent signal: optimize concentration and exposure time for each cell line, basal medium and target lineage, starting from a validated protocol and a dose-response study.\u003c\/li\u003e\n\u003cli\u003eLineage outcome depends on the starting cell state, cytokine combination, timing and culture conditions; BMP-4 acts together with WNT, Activin\/Nodal, FGF, VEGF and Notch signaling.\u003c\/li\u003e\n\u003cli\u003eBMP-4 and the BMP antagonist Noggin can be used at different stages to switch BMP signaling on and later off in a controlled workflow.\u003c\/li\u003e\n\u003cli\u003eFor organoid work (kidney, liver, lung and others), set dose and timing according to the developmental logic of the specific system.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eBMP-4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eBone Morphogenetic Protein 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHEK293 human cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eDisulfide-linked homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eTGF-β superfamily\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free manufacturing format\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized, carrier-protein free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInduction of alkaline phosphatase activity in ATDC-5 mouse chondrogenic cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEC50\u003c\/th\u003e\n\u003ctd\u003eApproximately 5 ng\/mL (representative ATDC-5 assay)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Hematopoietic Differentiation, 3D cell culture, Cell Reprogramming, Tissue Engineering\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eActivity is determined in ATDC-5 mouse chondrogenic cells, using alkaline phosphatase induction as the readout; in a representative assay the EC50 was approximately 5 ng\/mL. Each lot is tested for purity, endotoxin and biological activity before release.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile 4 mM HCl containing at least 0.1% human serum albumin or bovine serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently; do not vortex aggressively.\u003c\/li\u003e\n\u003cli\u003ePrepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eShore EM, Xu M, Shah PB, et al. (1998) The human bone morphogenetic protein 4 (BMP-4) gene: molecular structure and transcriptional regulation. Calcif Tissue Int 63:221-229. \u003ca href=\"https:\/\/doi.org\/10.1007\/s002239900518\" target=\"_blank\" rel=\"noopener\"\u003e10.1007\/s002239900518\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eGoldman DC, Hackenmiller R, Nakayama T, et al. (2006) Mutation of an upstream cleavage site in the BMP4 prodomain leads to tissue-specific loss of activity. Development 133:1933-1942. \u003ca href=\"https:\/\/doi.org\/10.1242\/dev.02368\" target=\"_blank\" rel=\"noopener\"\u003e10.1242\/dev.02368\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePerry JM, Harandi OF, Porayette P, et al. (2009) Maintenance of the BMP4-dependent stress erythropoiesis pathway in the murine spleen requires hedgehog signaling. Blood 113:911-918. \u003ca href=\"https:\/\/doi.org\/10.1182\/blood-2008-03-147892\" target=\"_blank\" rel=\"noopener\"\u003e10.1182\/blood-2008-03-147892\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eLian X, Hsiao C, Wilson G, et al. (2012) Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A 109:E1848-E1857. \u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.1200250109\" target=\"_blank\" rel=\"noopener\"\u003e10.1073\/pnas.1200250109\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641413927277,"sku":"HST-B4-0010","price":215.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641425887597,"sku":"HST-B4-0100","price":1536.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/bmp4-500x500.webp?v=1791572721","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-bmp-4-protein-bhp22200003","provider":"BioHippo","version":"1.0","type":"link"}