{"product_id":"recombinant-human-bmp-7-protein-bhp22200004","title":"Recombinant Human BMP-7 Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · TGF-β Superfamily\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human BMP-7 Protein\u003c\/strong\u003e (osteogenic protein 1, OP1) is a tag-free, full-length human BMP-7 expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a non-glycosylated homodimer. It is made in an animal-free, xeno-free format and lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eBMP-7 is used to study bone and cartilage formation, including differentiation of mesenchymal stem cells (MSCs) toward bone and cartilage, fracture-repair and tissue-engineering models, kidney development and bone disease. Its activity is confirmed by induction of alkaline phosphatase in the mouse chondrogenic cell line ATDC-5.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eBMP-7, also called osteogenic protein 1 (OP1), belongs to the transforming growth factor β (TGF-β) family, a group of more than 20 structurally related bone growth factors. It has an important role in cartilage and bone formation and also contributes to calcium regulation and kidney development.\u003c\/p\u003e\n\u003cp\u003eMature human BMP-7 is usually a homodimer of two antiparallel monomers of 139 amino acids. The monomers are linked by a disulfide bridge, and each one carries three intrachain disulfide bridges arranged in a cysteine-knot motif.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eRecombinant human BMP-7 is a research tool for bone, cartilage and stem-cell biology.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBone and cartilage formation studies, including tissue-engineering and regenerative-medicine models.\u003c\/li\u003e\n\u003cli\u003eDifferentiation of mesenchymal stem cells (MSCs) toward bone and cartilage.\u003c\/li\u003e\n\u003cli\u003eFracture-repair and bone-healing models.\u003c\/li\u003e\n\u003cli\u003eKidney development and function research.\u003c\/li\u003e\n\u003cli\u003eIncorporation into biomaterials to build bone and cartilage grafts for research.\u003c\/li\u003e\n\u003cli\u003eStudies of bone disease mechanisms and drug-target discovery.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eBMP-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eOP1 (osteogenic protein 1)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eNon-glycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eTGF-β family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e30–38 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInduction of alkaline phosphatase production in the ATDC-5 cell line (mouse chondrogenic cell line)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Tissue Engineering\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to induce alkaline phosphatase production in ATDC-5 cells, a mouse chondrogenic cell line.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual defrost freezer is recommended for storage.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eWang RN, Green J, Wang Z, et al. (2014) Bone Morphogenetic Protein (BMP) signaling in development and human diseases. Genes Dis 1:87-105. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.gendis.2014.07.005\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.gendis.2014.07.005\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eChen D, Zhao M, Mundy GR (2004) Bone morphogenetic proteins. Growth Factors 22:233-241. \u003ca href=\"https:\/\/doi.org\/10.1080\/08977190412331279890\" target=\"_blank\" rel=\"noopener\"\u003e10.1080\/08977190412331279890\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eCeleste AJ, Iannazzi JA, Taylor RC, et al. (1990) Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. Proc Natl Acad Sci U S A 87:9843-9847. \u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.87.24.9843\" target=\"_blank\" rel=\"noopener\"\u003e10.1073\/pnas.87.24.9843\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641417531757,"sku":"HST-B7-0010","price":108.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641426215277,"sku":"HST-B7-0100","price":769.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/BMP7-500x500.png?v=1791572728","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-bmp-7-protein-bhp22200004","provider":"BioHippo","version":"1.0","type":"link"}