{"product_id":"recombinant-human-bpgm-protein-his-tag-bhp10303223","title":"Recombinant Human BPGM Protein (His Tag)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc ul{margin:0 0 14px;padding-left:22px}\n.bhp-desc li{margin-bottom:4px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin:4px 6px 10px 0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px;display:inline-block;margin:3px 4px 3px 0}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin:0 6px 10px 0}\n\u003c\/style\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman BPGM Protein\u003c\/strong\u003e — also known as 2; 3-Bisphosphoglycerate Mutase Erythrocyte; 2; 3-Bisphosphoglycerate Synthase; 2; 3-Diphosphoglycerate Mutase; BPG-Depe — Bisphosphoglycerate Mutase (BPGM) is a member of the Phosphoglycerate Mutase family and BPG-Dependent PGAM subfamily. BPGM is a multifunctional enzyme. BPGM catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. It also has phosphoglycerate phosphomutase activity. BPGM plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Deficiency of BPGM increases the affinity of cells for oxygen and result in hemolytic anemia.\u003c\/p\u003e\n\u003cp\u003eResearch into Human BPGM Protein supports investigations in tumor biology and oncology signaling, with implications for oncology signaling.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003e\u003cspan class=\"bhp-cat-pill\"\u003eE. coli (Prokaryotic)\u003c\/span\u003e\u003cspan class=\"bhp-cat-pill\"\u003eC-His\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eProduced in \u003cstrong\u003eE. coli (Prokaryotic)\u003c\/strong\u003e, yielding a high-purity non-glycosylated form suitable for structural and biochemical applications.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003eC-His\u003c\/strong\u003e enables single-step affinity purification and can also serve as a handle for direct detection using anti-tag antibodies without target-specific reagents.\u003c\/p\u003e\n\u003cp\u003eExpression region: \u003cstrong\u003eSer2-Lys259\u003c\/strong\u003e.\u003c\/p\u003e\n\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight (Calculated)\u003c\/th\u003e\n\u003ctd\u003e31.0 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight (Observed SDS-PAGE)\u003c\/th\u003e\n\u003ctd\u003e30 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt; 95% as determined by reducing SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAffinity Tag\u003c\/th\u003e\n\u003ctd\u003eC-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression System\u003c\/th\u003e\n\u003ctd\u003eE. coli (Prokaryotic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin Level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eSupplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, pH 8.0.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReconstitution\u003c\/th\u003e\n\u003ctd\u003eNot Applicable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Conditions\u003c\/th\u003e\n\u003ctd\u003eStore at \u0026lt; -20°C, stable for 6 months. Please minimize freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShelf Life\u003c\/th\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTypical Lead Time\u003c\/th\u003e\n\u003ctd\u003e2-3 business days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAccession Number\u003c\/th\u003e\n\u003ctd\u003eP07738\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt ID\u003c\/th\u003e\n\u003ctd\u003eP07738\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression Region\u003c\/th\u003e\n\u003ctd\u003eSer2-Lys259\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003ePurity \u0026gt;95%\u003c\/span\u003e \u003cspan class=\"bhp-qc-badge\"\u003eEndotoxin Tested\u003c\/span\u003e\n\u003ch2\u003eBiological Activity\u003c\/h2\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003eActivity Validated\u003c\/span\u003e\n\u003cp\u003eNot validated for activity\u003c\/p\u003e\n\u003cp\u003ePerform a titration in your specific assay format to establish optimal working concentration. Include a previously validated lot as an in-house reference when qualifying new lots.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Intended Use\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle in accordance with good laboratory practice.\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003eResearch Use Only (RUO)\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eVendor\u003c\/strong\u003eElabscience Biotechnology Co Ltd\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003ePKSH032119\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003eHuman\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eUniProt\u003c\/strong\u003eP07738\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology Co Ltd","offers":[{"title":"10 ug","offer_id":53335890493805,"sku":"PKSH032119_10μg","price":88.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53335894196589,"sku":"PKSH032119_50μg","price":198.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/5Z1xq1.jpg?v=1778912914","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-bpgm-protein-his-tag-bhp10303223","provider":"BioHippo","version":"1.0","type":"link"}