{"product_id":"recombinant-human-chromodomain-helicase-dna-binding-protein-4-bhp11003868","title":"Recombinant Human Chromodomain Helicase DNA Binding Protein 4","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Human Chromodomain Helicase DNA Binding Protein 4\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eCHD4 is a member of a family of alleged chromodomain helicase-DNA-binding proteins. Biochemically, CHD4 is a component of the nucleosome transformation and deacetylase (NuRD) complex that takes part in transcription regulation. Autoantibodies targeting the CHD4 are a serologic feature of idiopathic inflammatory myopathies (IIM). In IIM Mi-2 antibodies are characterized by diagnostic sensitivity and specificity of approximately 4-18% and 98-100%, respectively. Moreover, anti-CHD4 antibodies are related to dermatomyositis (frequency up to 31%) and have a great positive predictive value for this type of disease subset. Anti-CHD4 are the only defined myositis-specific autoantibodies clearly focused to a nuclear target. An additional slightly outstanding feature of Mi-2 antibodies relates to their frequency in children, which is similar to that in adults.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eActivity \u0026amp; assay information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunological functions:\u003c\/strong\u003e 1. Binds IgG-type human auto-antibodies.\u003cbr\u003e2. Standard ELISA test (checkerboard analysis of positive\/negative sera panels); immunodot test with positive\/negative sera panels.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCoating concentration:\u003c\/strong\u003e 0.3-0.7 µg\/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"5 ug","offer_id":53038128234861,"sku":"pro-112-5UG","price":60.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53038434713965,"sku":"pro-112-20UG","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53038434746733,"sku":"pro-112-1MG","price":5200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-chd4-human-PRO-112.png?v=1782158290","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-chromodomain-helicase-dna-binding-protein-4-bhp11003868","provider":"BioHippo","version":"1.0","type":"link"}