{"product_id":"recombinant-human-collagen-type-iv-alpha-3-biotinylated-bhp11005754","title":"Recombinant Human Collagen Type IV Alpha 3, Biotinylated","description":"\u003cp\u003e\u003cstrong\u003eRecombinant Human Collagen Type IV Alpha 3, Biotinylated\u003c\/strong\u003e is supplied as a recombinant protein for in vitro research use.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\n\u003cp\u003eType IV collagen is a major structural component of basement membranes. It is a multimeric protein composed of 3 alpha subunits, which are encoded by 6 different genes, alpha 1 through alpha\u003cbr\u003e6. Each of these alpha subunits can form a triple helix structure with 2 other subunits to form type IV collagen. The Goodpasture syndrome is a condition in which autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are restricted basically to the non-collagenous C-terminal domain of the protein. There are numerous alternative transcripts that appear to be unique to the human COL4A3 and alternative splicing is limited to the six exons that encode this C-terminal domain. COL4A3 is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to the Alport syndrome are also situated within the exons that encode this C-terminal region. COL4A3 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Several exons of COL4A3 are interspersed with exons of an uncharacterized gene which is on the opposite strand.\u003c\/p\u003e\n\u003ch3\u003eProduct format\u003c\/h3\u003e\n\u003cp\u003eProvided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.\u003c\/p\u003e\n\u003ch3\u003eActivity \u0026amp; assay information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNote:\u003c\/strong\u003e It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunological functions:\u003c\/strong\u003e 1. Binds IgG type human auto antibodies.\u003cbr\u003e2. Functional Streptavidin based ELISA test (analysis of positive\/negative samples.)\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"50 ug","offer_id":53038172963181,"sku":"pro-2564-50UG","price":600.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53038525874541,"sku":"pro-2564-100UG","price":900.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53038525907309,"sku":"pro-2564-1MG","price":5200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-glomerular-basal-membrane-PRO-2564.png?v=1782158375","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-collagen-type-iv-alpha-3-biotinylated-bhp11005754","provider":"BioHippo","version":"1.0","type":"link"}