{"product_id":"recombinant-human-dcc-interacting-protein-13-alpha-appl1-bhp10507681","title":"Recombinant Human DCC-interacting protein 13-alpha (APPL1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eAPPL1\u003c\/strong\u003e from Homo sapiens (Human), produced in E.coli (region 1-709aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 1-709aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Adapter protein containing PH domain, PTB domain and leucine zipper motif 1 (Dip13-alpha). Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD\/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K\/Akt signaling in macrophages. Regulates TLR4 signaling in activated macrophages . Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling. Positive regulator of beta-catenin\/TCF-dependent transcription through direct interaction with RUVBL2\/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin\/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eQuantitative mapping of ligand\/receptor signaling to downstream phospho- and transcriptional programs.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eMultifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD\/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K\/Akt signaling in macrophages. Regulates TLR4 signaling in activated macrophages . Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling. Positive regulator of beta-catenin\/TCF-dependent transcription through direct interaction with RUVBL2\/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin\/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving APPL1\u003c\/li\u003e\n\u003cli\u003eBinding interaction studies (e.g., SPR\/BLI or plate-based binding formats)\u003c\/li\u003e\n\u003cli\u003eCell-based stimulation studies with downstream marker readouts (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB Q9UKG1 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q9UKG1 - NCBI Gene search: APPL1 — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=APPL1 - Ensembl search: APPL1 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=APPL1 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053421060461,"sku":"CSB-EP890917HU-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053563044205,"sku":"CSB-EP890917HU-100UG","price":578.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053563076973,"sku":"CSB-EP890917HU-20UG","price":306.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP890917HU-SDS.jpg?v=1772177701","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-dcc-interacting-protein-13-alpha-appl1-bhp10507681","provider":"BioHippo","version":"1.0","type":"link"}