{"product_id":"recombinant-human-diablo-homolog-mitochondrial-diablo-bhp10507654","title":"Recombinant Human Diablo homolog, mitochondrial (DIABLO)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eDIABLO\u003c\/strong\u003e from Homo sapiens (Human), produced in E.coli (region 56-239aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 56-239aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e GST (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Direct IAP-binding protein with low pI (Second mitochondria-derived activator of caspase) (Smac) (SMAC). Promotes apoptosis by activating caspases in the cytochrome c\/Apaf-1\/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6\/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP\/BIRC4 by promoting XIAP\/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP\/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP\/BIRC4 abundance.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eQuantitative mapping of ligand\/receptor signaling to downstream phospho- and transcriptional programs.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003ePromotes apoptosis by activating caspases in the cytochrome c\/Apaf-1\/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6\/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP\/BIRC4 by promoting XIAP\/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP\/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP\/BIRC4 abundance.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving DIABLO\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB Q9NR28 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q9NR28 - NCBI Gene search: DIABLO — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DIABLO - Ensembl search: DIABLO — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DIABLO - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053419815277,"sku":"CSB-EP865113HU-1MG","price":2062.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053560881517,"sku":"CSB-EP865113HU-100UG","price":480.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053560914285,"sku":"CSB-EP865113HU-20UG","price":256.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP865113HU-SDS.jpg?v=1772177695","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-diablo-homolog-mitochondrial-diablo-bhp10507654","provider":"BioHippo","version":"1.0","type":"link"}