{"product_id":"recombinant-human-dna-dc-du-editing-enzyme-apobec-3a-apobec3a-bhp10510361","title":"Recombinant Human DNA dC-\u003edU-editing enzyme APOBEC-3A (APOBEC3A)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eRecombinant Human DNA dC-\u0026gt;dU-editing enzyme APOBEC-3A (APOBEC3A) is a recombinant protein reagent derived from Homo sapiens (Human) and produced in Yeast. It is commonly used to support Signal Transduction research by enabling binding assays, assay development and protein–protein interaction studies in controlled in vitro settings.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 1-199aa. Region selection can focus on functional domains, improve solubility, or isolate interaction surfaces for targeted studies.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Yeast. Expression host can influence folding and the presence\/absence of post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eTag \/ fusion:\u003c\/strong\u003e C-terminal 6xHis-tagged. Tags can support purification and detection; evaluate potential tag effects when studying sensitive interactions.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight (reported):\u003c\/strong\u003e 24.5 kDa. Apparent size may vary with tags, processing, and gel conditions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWhen comparing results across batches or platforms, interpret signals in the context of construct design (region, tags) and expression host, especially for modification-dependent interactions.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eThe gene commonly associated with this target is \u003cstrong\u003eAPOBEC3A\u003c\/strong\u003e. APOBEC3A refers to a protein target that is studied across multiple biological contexts; annotations and nomenclature can vary by species and isoform. This product corresponds to the Homo sapiens (Human) sequence context, which can be important when comparing homologs or orthologs across model systems. For curated functional annotations, domains, and sequence features, consult primary databases (e.g., UniProt\/NCBI) and the recent literature for the specific organism and isoform.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eUsing recombinant proteins to enable quantitative binding measurements and reagent benchmarking.\u003c\/li\u003e \u003cli\u003eStudying domain- and isoform-specific effects in pathway models and interaction networks.\u003c\/li\u003e \u003cli\u003eDeveloping robust, reproducible assays that connect molecular readouts to cellular phenotypes.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eRelevance:\u003c\/strong\u003e DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.\u003c\/p\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eEnzyme activity assays and kinetics measurements with defined substrates\/cofactors.\u003c\/li\u003e \u003cli\u003eInhibitor, activator, or substrate screening in biochemical assay formats.\u003c\/li\u003e \u003cli\u003eStructure–function analysis to interpret how sequence changes impact catalytic performance.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eIn quantitative assay development, changes in binding or activity readouts are typically interpreted relative to appropriate negative\/positive controls and, where possible, orthogonal assay formats that support the same conclusion.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eRecombinant constructs may represent a defined region (domain) rather than the full-length protein; interpret results in the context of the expressed region.\u003c\/li\u003e \u003cli\u003eTag or fusion elements can aid purification and detection but may influence binding surfaces or oligomerization; consider tag controls when relevant.\u003c\/li\u003e \u003cli\u003eSpecies and isoform differences can affect interaction partners and post-translational modifications; align experimental controls to the intended biological context.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry for P31941 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P31941\/entry - NCBI Gene search (APOBEC3A) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=APOBEC3A - PubMed search (APOBEC3A) — NCBI — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=APOBEC3A - RCSB PDB search (APOBEC3A) — RCSB PDB — https:\/\/www.rcsb.org\/search?query=APOBEC3A - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53065317908845,"sku":"CSB-YP001921HU-1MG","price":2310.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53065487286637,"sku":"CSB-YP001921HU-100UG","price":516.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53065487319405,"sku":"CSB-YP001921HU-20UG","price":276.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-YP001921HU-SDS.jpg?v=1772476569","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-dna-dc-du-editing-enzyme-apobec-3a-apobec3a-bhp10510361","provider":"BioHippo","version":"1.0","type":"link"}