{"product_id":"recombinant-human-dual-specificity-mitogen-activated-protein-kinase-kinase-6-map2k6-bhp10504017","title":"Recombinant Human Dual specificity mitogen-activated protein kinase kinase 6 (MAP2K6)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Human Dual specificity mitogen-activated protein kinase kinase 6 (MAP2K6) is a recombinant protein reagent for research-use applications such as assay development, binding studies, and mechanistic experiments. It corresponds to \u003cstrong\u003eMAP2K6\u003c\/strong\u003e (Homo sapiens (Human)) and is intended for RUO workflows where a defined protein standard or functional input is needed.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Baculovirus (expression context can influence folding and PTMs).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression region:\u003c\/strong\u003e 1-334aa (region choice can affect activity and binding readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate(s)\/tag:\u003c\/strong\u003e N-terminal 6xHis-tagged (can support detection or purification depending on format).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 39.5 kDa (useful for interpreting gel migration and size-exclusion profiles).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eWhen comparing results across assays, consider that expression system and expressed region can alter glycosylation, disulfide formation, and oligomerization state, which may shift apparent potency or binding behavior in vitro.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eDual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3\/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3\/MKK3 and MAP2K6\/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6\/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6\/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6\/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6\/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eReagent standardization: using recombinant proteins as reference materials for quantitative calibration and cross-study comparability.\u003c\/li\u003e\n\u003cli\u003eInteraction-focused studies: mapping binding partners, affinity changes, and structure–function relationships across variants or domains.\u003c\/li\u003e\n\u003cli\u003eMulti-omic readouts: combining recombinant perturbations with transcript, protein, and functional endpoints to connect mechanism to phenotype.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAssay development and validation: use as a defined input or standard where protein identity is required.\u003c\/li\u003e\n\u003cli\u003eBinding studies: evaluate interaction strength and specificity using plate-based or biophysical formats.\u003c\/li\u003e\n\u003cli\u003eCell-response profiling: add protein to cultured cells and interpret downstream marker changes with appropriate controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpretation is most robust when signal changes are evaluated relative to matched controls (buffer-only, unrelated protein controls, or pathway controls) and when readouts are compared across dose and time.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eIsoforms and PTMs can influence binding and activity; ensure the expressed region and expression system match your experimental needs.\u003c\/li\u003e\n\u003cli\u003eSpecies differences may affect receptor binding or antibody recognition; confirm species\/source alignment with your model.\u003c\/li\u003e\n\u003cli\u003eUse concept-level controls such as negative controls (no protein), matrix controls, or orthogonal readouts to support conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt keyword search: https:\/\/www.uniprot.org\/uniprotkb?query=MAP2K6 - NCBI Gene search: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MAP2K6 - PubMed search: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MAP2K6 - Ensembl search: https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MAP2K6 - Reactome Pathway Browser: https:\/\/reactome.org\/content\/query?q=MAP2K6 --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053041967469,"sku":"CSB-BP013415HU-1MG","price":2710.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053187031405,"sku":"CSB-BP013415HU-100UG","price":1120.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053187064173,"sku":"CSB-BP013415HU-20UG","price":400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-BP013415HU-SDS.jpg?v=1772172862","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-dual-specificity-mitogen-activated-protein-kinase-kinase-6-map2k6-bhp10504017","provider":"BioHippo","version":"1.0","type":"link"}