{"product_id":"recombinant-human-epha8-bhp10807230","title":"Recombinant Human EphA8","description":"\u003ch2\u003eBackground\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eEphA8\u003c\/strong\u003e is used in \u003cstrong\u003eresearch use only (RUO)\u003c\/strong\u003e settings as a defined recombinant protein reagent. Bench researchers commonly leverage recombinant proteins to create controlled experimental conditions for mechanistic studies, assay development, interaction mapping, and quantitative benchmarking across model systems.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e EEK, EK3, HEK3, EPH- and ELK-related kinase, EPH- and ELK-related tyrosine kinase, EPH receptor A8.\u003c\/p\u003e\u003ch2\u003eBiological significance and function\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eEphA8\u003c\/strong\u003e is commonly investigated in RUO studies for its role in ligand recognition and receptor-mediated signaling. Recombinant receptor ectodomains or domains are often used to analyze binding specificity\/affinity, interrogate competitive interactions, and support structural or biophysical experiments.\u003c\/p\u003e\u003cp\u003eMechanistically, researchers often analyze how EphA8 participates in pathway networks through molecular interactions, localization, and regulated activity. Depending on the target class, this can involve receptor-mediated signaling, enzymatic catalysis, complex assembly, or structural organization that shapes downstream cellular phenotypes.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch relevance:\u003c\/strong\u003e RUO studies frequently connect EphA8 to perturbations such as immune stimulation, stress signaling, differentiation cues, metabolic remodeling, or engineered genetic modulation—then interpret downstream readouts using complementary pathway markers.\u003c\/p\u003e\u003ch2\u003eMolecular characteristics\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eMolecular features matter in RUO experiments:\u003c\/strong\u003e domain boundaries, oligomerization state, and PTM sensitivity can influence binding behavior, stability, and functional readouts in vitro.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConstruct \/ expression region:\u003c\/strong\u003e aa 31­542\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApprox. molecular weight:\u003c\/strong\u003e 65-80 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEndotoxin level:\u003c\/strong\u003e \u0026lt; 1 EU\/µg as determined by LAL test.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 6% Trehalose, 50 mM NaCl, 0.05% Tween 80, pH 5.0.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg\/ml by gently pipetting 2-3 times, don't vortex.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003ePTM considerations:\u003c\/strong\u003e For many extracellular ligands and receptor ectodomains, \u003cstrong\u003edisulfide bonds\u003c\/strong\u003e and \u003cstrong\u003eglycosylation\u003c\/strong\u003e can influence stability and binding. PTM dependence is target- and assay-specific. Eukaryotic expression can support native-like folding and certain PTMs, which may better match some receptor\/ligand assays.\u003c\/p\u003e\u003ch2\u003eExpression and purification strategy\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eExpression system:\u003c\/strong\u003e This protein is produced in a \u003cstrong\u003emammalian\u003c\/strong\u003e expression system, which can support native-like folding, disulfide bonding, and PTMs (e.g., glycosylation) that may be important for extracellular ligands, receptors, and secreted proteins in research assays.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePurification transparency (research credibility):\u003c\/strong\u003e In research-grade recombinant protein production, constructs are commonly purified via affinity and polishing steps (e.g., chromatography) to reduce contaminants and improve batch-to-batch consistency. When present, affinity tags (e.g., His\/GST\/Fc) can simplify purification; tag presence or removal can influence certain binding or structural assays.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eForm and handling context:\u003c\/strong\u003e Lyophilized proteins are frequently used in RUO labs to support stability during storage and shipment, while formulation components and reconstitution conditions can impact solubility and aggregation—important considerations when comparing studies across publications.\u003c\/p\u003e\u003ch2\u003eResearch interpretation\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eResearch interpretation:\u003c\/strong\u003e Receptor abundance, domain composition, and PTM state can influence binding and signaling outputs. When using recombinant \u003cstrong\u003eEphA8\u003c\/strong\u003e, researchers often consider construct boundaries (domain\/region), oligomerization, and PTM sensitivity as potential drivers of assay behavior.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eUsing recombinant protein as a defined reagent:\u003c\/strong\u003e recombinant EphA8 is commonly used as a quantitative input for assay calibration, antibody\/ligand binding studies, pathway reconstitution, and controlled perturbation experiments. Researchers often consider isoforms, fragments, or construct boundaries when comparing results across studies.\u003c\/p\u003e","brand":"Fine Test","offers":[{"title":"10 ug","offer_id":53014630367597,"sku":"Pr22611-10UG","price":130.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53014630400365,"sku":"Pr22611-50UG","price":390.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53014630433133,"sku":"Pr22611-500UG","price":2210.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53014630465901,"sku":"Pr22611-1MG","price":3324.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/E8_9B_8B_E7_99_BD_0d76d632-38ff-4439-9e8d-10849cb88afd.jpg?v=1770541607","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-epha8-bhp10807230","provider":"BioHippo","version":"1.0","type":"link"}