{"product_id":"recombinant-human-fgf-7-kgf-protein-bhp22200007","title":"Recombinant Human FGF-7 (KGF) Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human FGF-7 (KGF) Protein\u003c\/strong\u003e is keratinocyte growth factor expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e and supplied as a glycosylated homodimer. It is tag-free, animal-free and xeno-free, and is lyophilized from a 0.2 µm-filtered PBS solution without carrier protein, with purity \u0026gt;95% by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eFGF-7 is an epithelium-selective growth factor that acts through FGFR2b. Typical research uses are keratinocyte and epithelial proliferation, organoid culture and staged endoderm differentiation, branching morphogenesis and epithelial repair models. Expression in human cells supports mammalian processing of the protein, and three peer-reviewed studies report use of this FGF-7.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eFGF-7, also called keratinocyte growth factor (KGF), is a fibroblast growth factor made mainly by mesenchymal cells. It acts as a paracrine signal on neighboring epithelial cells, primarily through the FGFR2b receptor isoform, and activates MAPK, PI3K–AKT and related pathways that support proliferation and survival.\u003c\/p\u003e\n\u003cp\u003eFGF-7 research spans keratinocyte biology and epithelial repair, branching morphogenesis, development of the lung and pancreas, and organoid and staged endoderm differentiation models. The response depends on FGFR2b expression, developmental stage, matrix and companion signals.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eFGF-7 is used wherever FGFR2b-positive epithelial cells need a paracrine growth or survival signal. The table shows typical placements; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of FGF-7\u003c\/th\u003e\n\u003cth\u003eCompanion context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEpithelial proliferation\u003c\/td\u003e\n\u003ctd\u003eDirect FGFR2b stimulation\u003c\/td\u003e\n\u003ctd\u003eDefined serum context\u003c\/td\u003e\n\u003ctd\u003eCell count, EdU\/Ki-67, pERK and viability\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePancreatic \/ endoderm differentiation\u003c\/td\u003e\n\u003ctd\u003eStage-specific support of epithelial progenitors\u003c\/td\u003e\n\u003ctd\u003eActivin A, retinoic acid and other protocol factors\u003c\/td\u003e\n\u003ctd\u003ePDX1, NKX6.1, organoid morphology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBranching morphogenesis\u003c\/td\u003e\n\u003ctd\u003eParacrine epithelial growth signal\u003c\/td\u003e\n\u003ctd\u003eMatrix, with FGF10\/EGF controls\u003c\/td\u003e\n\u003ctd\u003eBranch number, epithelial markers and FGFR2b\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRepair models\u003c\/td\u003e\n\u003ctd\u003eEpithelial survival and proliferation\u003c\/td\u003e\n\u003ctd\u003eInjury model and time course\u003c\/td\u003e\n\u003ctd\u003eClosure, apoptosis, proliferation and barrier function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the responsive cell stage and confirm FGFR2b expression or pathway competence before adding FGF-7.\u003c\/li\u003e\n\u003cli\u003eBegin with conditions from a validated protocol and titrate FGF-7 in the actual cells, medium and endpoint; no numerical EC50 is given for this product.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion factors such as EGF, FGF-10 or Activin A, since signal order and duration can change the outcome.\u003c\/li\u003e\n\u003cli\u003eInclude no-factor, vehicle and pathway-response controls, and measure phenotype and function rather than cell number alone.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eFGF-7 (also known as KGF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eKGF; keratinocyte growth factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated homodimer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eFibroblast growth factor (FGF) family\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ Xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e17–30 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of 4MBr-5 monkey epithelial cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, Cell Proliferation, 3D cell culture, Cell Migration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by stimulation of 4MBr-5 monkey epithelial cell proliferation.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare aliquots.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eFinch PW, Rubin JS, Miki T, et al. (1989) Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth. Science 245:752-755. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.2475908\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.2475908\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinch PW, Rubin JS (2004) Keratinocyte growth factor\/fibroblast growth factor 7, a homeostatic factor with therapeutic potential for epithelial protection and repair. Adv Cancer Res 91:69-136. \u003ca href=\"https:\/\/doi.org\/10.1016\/S0065-230X(04)91003-2\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/S0065-230X(04)91003-2\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641418285421,"sku":"HST-F7-0010","price":112.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641426674029,"sku":"HST-F7-0100","price":779.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FGF7-500x500.png?v=1791572743","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-fgf-7-kgf-protein-bhp22200007","provider":"BioHippo","version":"1.0","type":"link"}