{"product_id":"recombinant-human-filamin-b-flnb-t2177a-v2354a-r2374a-partial-bhp10515724","title":"Recombinant Human Filamin-B (FLNB) (T2177A,V2354A,R2374A), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Human Filamin-B (FLNB) (T2177A,V2354A,R2374A), partial is a recombinant protein preparation derived from Homo sapiens (Human). It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 2091-2496aa(T2177A,V2354A,R2374A).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e C-terminal 6xHis-tagged; Liquid or Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 49.5 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eConnects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eDomain- and isoform-aware assay design to improve biological interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003eQuantitative workflows emphasizing calibration standards, spike-in controls, and cross-lot comparability.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding\/kinetics profiling (SPR\/BLI) to connect biochemical interactions with cellular phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrepare aliquots of FLNB for reproducible in vitro assays (minimize freeze–thaw).\u003c\/li\u003e\n\u003cli\u003eUse FLNB as a calibration standard in quantitative assays (standard curve setup).\u003c\/li\u003e\n\u003cli\u003eMeasure binding interactions to FLNB by SPR\/BLI (kinetic profiling in vitro).\u003c\/li\u003e\n\u003cli\u003eGenerate antibodies to FLNB and benchmark specificity in ELISA\/WB (control samples).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider species- and isoform-specific differences when comparing results across models or homologs.\u003c\/li\u003e\n\u003cli\u003eFor quantitative assays, include appropriate negative controls and matrix-matched spike-in concepts to assess non-specific signal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (O75369) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/O75369 - NCBI Gene search (FLNB) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FLNB - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FLNB - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059294593389,"sku":"CSB-EP008725HU1(M)-1MG","price":1812.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059432644973,"sku":"CSB-EP008725HU1(M)-100UG","price":419.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059432677741,"sku":"CSB-EP008725HU1(M)-20UG","price":224.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP008725HU1_M_-SDS.jpg?v=1772280322","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-filamin-b-flnb-t2177a-v2354a-r2374a-partial-bhp10515724","provider":"BioHippo","version":"1.0","type":"link"}