{"product_id":"recombinant-human-fxyd-domain-containing-ion-transport-regulator-3-fxyd3-partial-bhp10506440","title":"Recombinant Human FXYD domain-containing ion transport regulator 3 (FXYD3), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eFXYD3\u003c\/strong\u003e from Homo sapiens (Human), produced in E.coli (region 21-38aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 21-38aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His, SUMO (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Chloride conductance inducer protein Mat-8 (Mammary tumor 8 kDa protein) (Phospholemman-like) (Sodium\/potassium-transporting ATPase subunit FXYD3) ( MAT8) (PLML). Associates with and regulates the activity of the sodium\/potassium-transporting ATPase (NKA) which transports Na(+) out of the cell and K(+) into the cell.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eActivity assay development for kinetics, substrate scope, and inhibitor\/activator profiling.\u003c\/li\u003e\n\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eAssociates with and regulates the activity of the sodium\/potassium-transporting ATPase which transports Na+ out of the cell and K+ into the cell . Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1. Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving FXYD3\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB Q14802 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q14802 - NCBI Gene search: FXYD3 — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FXYD3 - Ensembl search: FXYD3 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FXYD3 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053384458605,"sku":"CSB-EP622789HU-1MG","price":2466.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053490364781,"sku":"CSB-EP622789HU-100UG","price":578.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053490397549,"sku":"CSB-EP622789HU-20UG","price":306.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP622789HU-SDS.jpg?v=1772177570","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-fxyd-domain-containing-ion-transport-regulator-3-fxyd3-partial-bhp10506440","provider":"BioHippo","version":"1.0","type":"link"}