{"product_id":"recombinant-human-g-protein-coupled-receptor-family-c-group-5-member-d-gprc5d-vlps-active-bhp10509498","title":"Recombinant Human G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product consists of virus-like particles (VLPs) displaying Human G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active) derived from Homo sapiens (Human). VLP-based presentation can help maintain membrane topology and conformational epitopes for multi-pass proteins, supporting reagent development and binding-focused research. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–345 (345 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 7TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Mammalian cell. Mammalian expression can support more native-like folding and post-translational modifications for complex membrane proteins, which may be important for conformation-sensitive binding studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Homo sapiens (Human). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt Q9NZD1. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eHuman G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active) is a membrane-associated protein from Homo sapiens. Available annotations suggest it gprc5d in mm cells makes this gene and its encoded surface protein as promising markers for monitoring the tumor load and hopefully also as targets for antimyeloma antibodies. pmid: 23510526 overexpression of g protein-coupled receptor 5d in the bone marrow is associated with poor prognosis in patients with multiple myeloma. pmid: 22591013. Many membrane proteins participate in transport, signaling, cell–cell interactions, or host–pathogen processes. For less-characterized entries, curated database annotations (e.g., UniProt) and domain predictions provide useful starting points for hypothesis generation. Also reported as: (GPRC5D)(G-protein coupled receptor family C group 5 member D).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIntegrating domain prediction, topology mapping, and comparative genomics to refine functional hypotheses for membrane proteins.\u003c\/li\u003e\n\u003cli\u003eUsing structural and biophysical methods (including stabilized constructs and membrane mimetics) to probe conformation and interactions.\u003c\/li\u003e\n\u003cli\u003eApplying single-cell and spatial omics to understand when and where membrane proteins are expressed and how that links to phenotype.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAntigen production for antibody generation, epitope mapping, or binder screening against defined regions.\u003c\/li\u003e\n\u003cli\u003eBiochemical interaction studies (protein–protein or protein–lipid) that inform pathway placement and mechanism.\u003c\/li\u003e\n\u003cli\u003eComparative studies of orthologs\/variants to explore conserved motifs and potential functional differences.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eVLP display considerations:\u003c\/strong\u003e VLP-based presentation can enrich for native-like membrane topology, but accessibility of specific loops\/epitopes can still vary with particle composition and protein orientation. Interpreting binding data benefits from using multiple controls and orthogonal readouts.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Human G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active) (Q9NZD1) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/Q9NZD1\/entry\n- NCBI Gene search: GPRC5D Homo sapiens — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GPRC5D%20Homo%20sapiens\n- PubMed search: GPRC5D review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GPRC5D%20review\n- InterPro search: GPRC5D — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/GPRC5D\/\n- Ensembl Gene Summary: GPRC5D (Homo_sapiens) — Ensembl — https:\/\/www.ensembl.org\/Homo_sapiens\/Gene\/Summary?g=GPRC5D\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n- PubMed search: virus-like particles membrane protein display review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=virus-like%20particles%20membrane%20protein%20display%20review\n--\u003e","brand":"CUSABIO TECHONOLOGY LLC","offers":[{"title":"1 mg","offer_id":53207328620909,"sku":"CSB-MP882153HU-1MG","price":5891.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53320595767661,"sku":"CSB-MP882153HU-100UG","price":1112.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320595800429,"sku":"CSB-MP882153HU-20UG","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-MP882153HU-WB.jpg?v=1778623133","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-g-protein-coupled-receptor-family-c-group-5-member-d-gprc5d-vlps-active-bhp10509498","provider":"BioHippo","version":"1.0","type":"link"}