{"product_id":"recombinant-human-granulocyte-colony-stimulating-factor-rhug-csf-bhp11300345","title":"Recombinant Human Granulocyte Colony Stimulating Factor (rHuG-CSF)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein\u003c\/span\u003e\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eGranulocyte colony-stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ, and it plays a central role in regulating neutrophil production during steady-state granulopoiesis and in emergency responses to infection.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression System: E. coli\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eRecombinant human G-CSF is expressed in Escherichia coli as a single non-glycosylated polypeptide chain containing 174 amino acids with an approximate molecular weight of 18.7 kDa. The protein is purified to \u0026gt;98% as determined by SDS-PAGE and HPLC analyses and is supplied as a sterile-filtered, white lyophilized powder.\u003c\/p\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eSource\u003c\/th\u003e\n\u003ctd\u003eEscherichia coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAmino Acid Sequence\u003c\/th\u003e\n\u003ctd style=\"word-break:break-all\"\u003eTPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;98% by SDS-PAGE and HPLC analyses.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin Level\u003c\/th\u003e\n\u003ctd\u003eLess than 1EU\/mg of rHuG-CSF as determined by LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2mm filtered concentrated  solution in 10mM sodium acetate buffer pH 4.0.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePhysical Appearance\u003c\/th\u003e\n\u003ctd\u003eSterile Filtered White lyophilized (freeze-dried) powder.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReconstitution\u003c\/th\u003e\n\u003ctd\u003eWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg\/mL. Stock solutions should be apportioned into working aliquots and stored at \u0026lt;-20°C. Further dilutions should be made in appropriate buffered solutions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eThis lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze\/thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eFunctional Activity\u003c\/h2\u003e\n\u003cp\u003eFully biologically active when compared to standard. The ED50, as calculated by the dose-dependent proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake), is less than 0.1 ng\/mL, corresponding to a specific activity of \u0026gt;1 × 10\u003csup\u003e8\u003c\/sup\u003e IU\/mg.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eThis product is for research use only (RUO) and is not intended for human or veterinary diagnostic or therapeutic use. Handle using standard laboratory safety practices.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eExplore additional recombinant cytokines and growth factors from Bioworld Technology Inc, or contact us for matched antibodies and ELISA kits for G-CSF research.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Bioworld Technology Inc","offers":[{"title":"2 ug","offer_id":53719339008365,"sku":"PR1034-2UG","price":163.0,"currency_code":"USD","in_stock":true},{"title":"10 ug","offer_id":53719339106669,"sku":"PR1034-10UG","price":307.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53719339139437,"sku":"PR1034-1MG","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/nopic_e016dab2-5237-4fb0-a230-e02dbfa3331d.jpg?v=1786566009","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-granulocyte-colony-stimulating-factor-rhug-csf-bhp11300345","provider":"BioHippo","version":"1.0","type":"link"}