{"product_id":"recombinant-human-herpesvirus-6b-g-protein-coupled-receptor-homolog-u51-u51-bhp10510467","title":"Recombinant Human herpesvirus 6B G-protein coupled receptor homolog U51 (U51)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Human herpesvirus 6B (strain Z29) (HHV-6 variant B) (Human B lymphotropic virus): Human herpesvirus 6B G-protein coupled receptor homolog U51 (U51) corresponding to amino acids 1–301. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–301 (301 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 7TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Human herpesvirus 6B (strain Z29) (HHV-6 variant B) (Human B lymphotropic virus). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt P52542. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eHuman herpesvirus 6B G-protein coupled receptor homolog U51 (U51) is described as a G protein-coupled receptor (GPCR), a large family of multi-pass membrane proteins that couple extracellular cues to intracellular signaling through heterotrimeric G proteins and other effectors. Many GPCRs are 'orphan' receptors with incompletely defined endogenous ligands, making them active areas for receptor deorphanization and chemical biology. Because GPCRs are conformationally dynamic and embedded in membranes, constructs and display formats can influence epitope accessibility and functional readouts.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDeorphanization and ligand discovery using high-throughput signaling assays, β-arrestin recruitment, and chemoproteomic approaches.\u003c\/li\u003e\n\u003cli\u003eStructure-guided GPCR research (cryo-EM and stabilized constructs) to map active\/inactive conformations and allosteric sites.\u003c\/li\u003e\n\u003cli\u003eBiased signaling and receptor trafficking studies that connect distinct signaling pathways to specific cellular phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAntibody or binder generation against extracellular loops\/domains to support receptor detection and localization studies.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding or interaction studies (e.g., ligand\/binder screening) where defined receptor fragments or displayed receptors aid comparability.\u003c\/li\u003e\n\u003cli\u003eCell biology studies of receptor expression, internalization, and pathway activation in engineered systems (interpretation focused, no protocols).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Human herpesvirus 6B G-protein coupled receptor homolog U51 (U51) (P52542) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P52542\/entry\n- NCBI Gene search: U51 Human herpesvirus 6B — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=U51%20Human%20herpesvirus%206B\n- PubMed search: U51 review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=U51%20review\n- InterPro search: U51 — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/U51\/\n- Ensembl Gene Summary: U51 (Homo_sapiens) — Ensembl — https:\/\/www.ensembl.org\/Homo_sapiens\/Gene\/Summary?g=U51\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n- Guide to PHARMACOLOGY (GPCR resource) — IUPHAR\/BPS — https:\/\/www.guidetopharmacology.org\/\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207329505645,"sku":"CSB-CF347336HKA-100UG","price":2781.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320597700973,"sku":"CSB-CF347336HKA-20UG","price":1668.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF347336HKA-SDS.jpg?v=1778623138","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-herpesvirus-6b-g-protein-coupled-receptor-homolog-u51-u51-bhp10510467","provider":"BioHippo","version":"1.0","type":"link"}