{"product_id":"recombinant-human-interleukin-10-receptor-subunit-alpha-il10ra-partial-bhp10515261","title":"Recombinant Human Interleukin-10 receptor subunit alpha (IL10RA), partial","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Human Interleukin-10 receptor subunit alpha (IL10RA), partial is a recombinant protein preparation derived from Homo sapiens (Human). It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 22-235aa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e Mammalian cell (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e C-terminal 10xHis-tagged; Liquid or Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 25.9 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eCell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eDomain- and isoform-aware assay design to improve biological interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003eQuantitative workflows emphasizing calibration standards, spike-in controls, and cross-lot comparability.\u003c\/li\u003e\n\u003cli\u003eIn vitro binding\/kinetics profiling (SPR\/BLI) to connect biochemical interactions with cellular phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStimulate cultured cells with IL10RA (dose–response + time-course) and collect lysates.\u003c\/li\u003e\n\u003cli\u003eMeasure downstream markers after IL10RA treatment (WB\/qPCR\/ELISA readouts).\u003c\/li\u003e\n\u003cli\u003eQuantify IL10RA using standard curves in plate-based assays (assay development).\u003c\/li\u003e\n\u003cli\u003eRun receptor binding kinetics for IL10RA by SPR\/BLI (in vitro profiling).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConsider species- and isoform-specific differences when comparing results across models or homologs.\u003c\/li\u003e\n\u003cli\u003eFor quantitative assays, include appropriate negative controls and matrix-matched spike-in concepts to assess non-specific signal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (Q13651) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/Q13651 - NCBI Gene search (IL10RA) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IL10RA - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IL10RA - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059283845485,"sku":"CSB-MP621688HUd7-1MG","price":2264.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059414163821,"sku":"CSB-MP621688HUd7-100UG","price":294.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059414196589,"sku":"CSB-MP621688HUd7-20UG","price":118.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-MP621688HUd7-SDS.jpg?v=1772280325","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-interleukin-10-receptor-subunit-alpha-il10ra-partial-bhp10515261","provider":"BioHippo","version":"1.0","type":"link"}