{"product_id":"recombinant-human-leukocyte-antigen-cd37-cd37-bhp10507061","title":"Recombinant Human Leukocyte antigen CD37 (CD37)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis product is a recombinant protein derived from Homo sapiens (Human): Human Leukocyte antigen CD37 (CD37) corresponding to amino acids 1–281. Defined recombinant constructs are commonly used as antigens or biochemical tools for antibody generation, interaction studies, and assay development. This material is supplied for research use only (RUO).\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e Amino acids 1–281 (281 aa) from the annotated sequence. Region choice can affect folding, solubility, and which epitopes are represented.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransmembrane architecture:\u003c\/strong\u003e Annotated as 4TM. Predicted topology influences detergent\/lipid dependence, epitope accessibility (extracellular vs cytosolic loops), and how results translate to full-length proteins in membranes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e in vitro E.coli expression system. Bacterial expression typically yields non-glycosylated protein and may require careful interpretation for eukaryotic membrane proteins where post-translational modifications can influence conformation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource species:\u003c\/strong\u003e Homo sapiens (Human). Ortholog differences can affect epitope conservation and functional interpretation across model systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReference accession:\u003c\/strong\u003e UniProt P11049. Curated annotations and sequence features in public databases can help interpret domains, motifs, and known isoforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMembrane proteins can be challenging analytes because conformation and interactions depend on the surrounding membrane environment. When using a recombinant region rather than a native membrane preparation, interpret binding and activity-oriented data in light of the construct boundaries, predicted topology, and expression host.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eHuman Leukocyte antigen CD37 (CD37) (also known as CD37\/TSPAN26) is a leukocyte surface tetraspanin with four predicted transmembrane segments. Tetraspanins organize membrane microdomains and can modulate receptor signaling, adhesion, and trafficking, particularly in immune cells. CD37 is frequently studied in B-cell biology and immune regulation, where surface expression and protein–protein interactions are central to interpretation. Also reported as: Tetraspanin-26 (Tspan-26) (CD37) (TSPAN26).\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eB-cell and tumor immunology research examining how tetraspanin microdomains tune signaling thresholds and cell–cell interactions.\u003c\/li\u003e\n\u003cli\u003eTarget discovery and reagent development for CD37 in immune-cell phenotyping and translational research settings (RUO).\u003c\/li\u003e\n\u003cli\u003eExtracellular vesicle and membrane-proteomics studies that profile tetraspanin-enriched compartments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGeneration and testing of antibodies\/binders to support flow-based detection or immunoassays (conceptual use, no protocols).\u003c\/li\u003e\n\u003cli\u003eStudies of CD37 surface abundance and redistribution during activation, differentiation, or perturbation.\u003c\/li\u003e\n\u003cli\u003eProtein interaction mapping within tetraspanin webs to interpret signaling or trafficking changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWhen interpreting signals from binding or detection assays, changes may reflect altered abundance, localization, or accessibility of the targeted region rather than changes in intrinsic activity. Pairing recombinant-protein results with cellular context (e.g., overexpression\/knockdown comparisons or orthogonal readouts) can strengthen conclusions without relying on any single assay format.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIsoforms, sequence variants, and proteolytic processing can change which extracellular or cytosolic regions are present and therefore which epitopes are detected.\u003c\/li\u003e\n\u003cli\u003ePost-translational modifications (e.g., glycosylation, disulfide bonding) and the membrane environment can influence conformation and binding; this can differ by expression system and sample type.\u003c\/li\u003e\n\u003cli\u003eUse appropriate negative\/positive control concepts (e.g., knockout\/knockdown or overexpression controls, orthogonal antibodies\/assays, and matched species\/ortholog controls) to support specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein considerations:\u003c\/strong\u003e Because E. coli does not perform most eukaryotic glycosylation and certain processing events, some epitopes or activities that depend on these features may not be fully represented. For many workflows (e.g., antibody generation or domain-focused binding studies), a well-defined region is still highly useful, especially when paired with orthogonal validation in cells or membranes.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry for Human Leukocyte antigen CD37 (CD37) (P11049) — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P11049\/entry\n- NCBI Gene search: CD37 Homo sapiens — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CD37%20Homo%20sapiens\n- PubMed search: CD37 review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CD37%20review\n- InterPro search: CD37 — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/CD37\/\n- Ensembl Gene Summary: CD37 (Homo_sapiens) — Ensembl — https:\/\/www.ensembl.org\/Homo_sapiens\/Gene\/Summary?g=CD37\n- InterPro topic search: transmembrane protein — EMBL-EBI — https:\/\/www.ebi.ac.uk\/interpro\/search\/text\/transmembrane%20protein\/\n- PubMed search: membrane protein expression in E. coli review — NIH\/NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=membrane%20protein%20expression%20E.%20coli%20review\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 ug","offer_id":53207325147501,"sku":"CSB-CF004928HU-100UG","price":1751.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53320593080685,"sku":"CSB-CF004928HU-20UG","price":1071.2,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-CF004928HU-SDS.jpg?v=1778623122","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-leukocyte-antigen-cd37-cd37-bhp10507061","provider":"BioHippo","version":"1.0","type":"link"}