{"product_id":"recombinant-human-neutrophil-elastase-elane-biotinylated-bhp10508150","title":"Recombinant Human Neutrophil elastase (ELANE), Biotinylated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis Recombinant Protein provides recombinant \u003cstrong\u003eELANE\u003c\/strong\u003e from Homo sapiens (Human), produced in E.coli (region 30-267aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRegion:\u003c\/strong\u003e 30-267aa (domain boundaries can affect binding\/activity readouts).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression host:\u003c\/strong\u003e E.coli (may differ from native PTMs\/processing).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag(s):\u003c\/strong\u003e His, MBP (supports purification\/detection; consider tag effects in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eAlso reported as Bone marrow serine protease Elastase-2 Human leukocyte elastase Medullasin PMN elastase. Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). Catalytic activity Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa \u0026gt; Ala-|-Xaa. EC:3.4.21.37.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eUse of recombinant standards to improve assay calibration and cross-study comparability.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eModifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). Catalytic activity Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa \u0026gt; Ala-|-Xaa. EC:3.4.21.37\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eStandard curve or spike-in reference for quantitative assays involving ELANE\u003c\/li\u003e\n\u003cli\u003eBinding and specificity benchmarking for detection reagents (conceptual)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombinant constructs may not capture all native isoforms or PTMs.\u003c\/li\u003e\n\u003cli\u003eConsider tag- or host-related effects when interpreting binding or activity.\u003c\/li\u003e\n\u003cli\u003eUse appropriate blanks and matrix\/control concepts to separate signal from background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB P08246 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P08246 - NCBI Gene search: ELANE — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ELANE - Ensembl search: ELANE — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ELANE - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ - NCBI Bookshelf — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53053435609453,"sku":"CSB-EP007587HU-B-1MG","price":2174.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53053591552365,"sku":"CSB-EP007587HU-B-100UG","price":502.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53053591585133,"sku":"CSB-EP007587HU-B-20UG","price":268.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP007587HU-B-SDS.jpg?v=1772177750","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-neutrophil-elastase-elane-biotinylated-bhp10508150","provider":"BioHippo","version":"1.0","type":"link"}