{"product_id":"recombinant-human-noggin-protein-bhp22200015","title":"Recombinant Human Noggin Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · BMP Antagonist\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant Human Noggin Protein\u003c\/strong\u003e is a full-length, glycosylated monomer expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. Noggin is a secreted antagonist of bone morphogenetic proteins (BMPs). The protein is made in an animal-free, xeno-free and tag-free format and supplied lyophilized without carrier protein, at \u0026gt;95% purity by SDS-PAGE and endotoxin \u0026lt;0.5 EU\/µg.\u003c\/p\u003e\n\u003cp\u003eResearchers use Noggin to block extracellular BMP signaling during neural induction and neural progenitor work, to keep a low-BMP environment in intestinal, gastric, neural and other organoid systems, and to study BMP-dependent patterning of neural, mesodermal and skeletal tissues. It also serves as a defined antagonist for titrating BMP-2\/BMP-4 responses in signaling assays. Function is confirmed in a cell-based BMP-4 antagonism assay.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eNoggin is a secreted cystine-knot protein that binds selected BMPs, most notably BMP-2 and BMP-4, and prevents them from reaching their type I and type II receptors. Less BMP–receptor engagement means less SMAD1\/5\/8 activation, which shifts cell-fate decisions in a way that depends strongly on developmental stage and context.\u003c\/p\u003e\n\u003cp\u003eIn vivo, Noggin has key roles in neural patterning, skeletal development and the formation of cartilage and joints. In culture it is not a universal differentiation factor: the outcome depends on the endogenous BMP level, Noggin concentration, matrix, cell state and companion pathways such as FGF, EGF, WNT and Activin\/Nodal.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eNoggin is used wherever BMP signaling needs to be reduced at a defined stage, from neural induction to organoid expansion. The table shows where it is typically placed in a workflow; it is a planning guide, not a universal protocol.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of Noggin\u003c\/th\u003e\n\u003cth\u003eCompanion signals and context\u003c\/th\u003e\n\u003cth\u003eSuggested readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeural induction\u003c\/td\u003e\n\u003ctd\u003eLowers BMP-dependent signals toward non-neural fates\u003c\/td\u003e\n\u003ctd\u003eOften combined with TGF-β\/Activin\/Nodal inhibition in dual-SMAD protocols\u003c\/td\u003e\n\u003ctd\u003ePAX6, SOX1, NESTIN, neural rosettes and lineage competence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganoid expansion\u003c\/td\u003e\n\u003ctd\u003eKeeps a tissue-specific low-BMP niche\u003c\/td\u003e\n\u003ctd\u003eEGF, FGF, WNT\/R-spondin or tissue-specific factors\u003c\/td\u003e\n\u003ctd\u003eOrganoid-forming efficiency, morphology, lineage markers and function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBMP signaling assay\u003c\/td\u003e\n\u003ctd\u003eExtracellular antagonist\u003c\/td\u003e\n\u003ctd\u003eBMP-2 or BMP-4 dose-response control\u003c\/td\u003e\n\u003ctd\u003epSMAD1\/5\/8, ID genes, alkaline phosphatase and reporter activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSkeletal\/chondrogenic research\u003c\/td\u003e\n\u003ctd\u003eModulates BMP-driven differentiation\u003c\/td\u003e\n\u003ctd\u003eMatrix and stage-specific chondrogenic signals\u003c\/td\u003e\n\u003ctd\u003eALP, SOX9, COL2A1, mineralization and morphology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDefine the developmental stage and confirm that the target cells depend on BMP signaling at that stage.\u003c\/li\u003e\n\u003cli\u003eStart from a validated or internally qualified concentration, then run a dose response for the cell source, basal medium and readout.\u003c\/li\u003e\n\u003cli\u003eControl timing and companion factors, since signal order and duration change the outcome; include no-factor, vehicle and BMP-response controls.\u003c\/li\u003e\n\u003cli\u003eAssess identity, viability, signaling and function rather than cell number alone. No universal working concentration or EC50 applies without lot- or protocol-specific evidence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eNoggin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStructure\u003c\/th\u003e\n\u003ctd\u003eGlycosylated monomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein family\u003c\/th\u003e\n\u003ctd\u003eSecreted cystine-knot BMP antagonist\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression region\u003c\/th\u003e\n\u003ctd\u003eFull length\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free \/ xeno-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003e65 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eInhibition of BMP-4-induced alkaline phosphatase in ATDC-5 cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Differentiation, Immunotherapy, 3D cell culture, Cell Signaling Studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of Noggin to inhibit BMP-4-induced alkaline phosphatase production in ATDC-5 mouse chondrogenic cells.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently and prepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles; a manual-defrost freezer is recommended.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eGroppe J, et al. (2002) Structural basis of BMP signalling inhibition by the cystine knot protein Noggin. Nature 420:636-42. \u003ca href=\"https:\/\/doi.org\/10.1038\/nature01245\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nature01245\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eBrunet LJ, et al. (1998) Noggin, cartilage morphogenesis, and joint formation in the mammalian skeleton. Science 280:1455-7. \u003ca href=\"https:\/\/doi.org\/10.1126\/science.280.5368.1455\" target=\"_blank\" rel=\"noopener\"\u003e10.1126\/science.280.5368.1455\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eChambers SM, et al. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol 27:275-80. \u003ca href=\"https:\/\/doi.org\/10.1038\/nbt.1529\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nbt.1529\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eSato T, Vries RG, Snippert HJ, et al. (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459:262-265. \u003ca href=\"https:\/\/doi.org\/10.1038\/nature07935\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nature07935\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eMcCracken KW, Catá EM, Crawford CM, et al. (2014) Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature 516:400-404. \u003ca href=\"https:\/\/doi.org\/10.1038\/nature13863\" target=\"_blank\" rel=\"noopener\"\u003e10.1038\/nature13863\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641419956589,"sku":"HST-NG-0010","price":109.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641429066093,"sku":"HST-NG-0100","price":758.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/noggin-500x500.png?v=1791572791","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-noggin-protein-bhp22200015","provider":"BioHippo","version":"1.0","type":"link"}