{"product_id":"recombinant-human-papillomavirus-type-18-minor-capsid-protein-l2-l2-bhp10513967","title":"Recombinant Human papillomavirus type 18 Minor capsid protein L2 (L2)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eRecombinant Human papillomavirus type 18 Minor capsid protein L2 (L2) is a recombinant protein preparation derived from Human papillomavirus type 18. It is commonly used as a defined reagent for assay development, binding studies, and analytical controls where consistent protein specifications are required.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 1-462aa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli (may influence folding and post-translational modifications).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTag\/format:\u003c\/strong\u003e C-terminal 6xHis-tagged; Liquid or Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExpected size:\u003c\/strong\u003e 56.5 kDa (as provided).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 85% as determined by SDS-PAGE.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eRegion choice, expression system, and tag\/format can influence folding, post-translational modifications, and interaction behavior in downstream assays.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eMinor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, L2 escorts the genomic DNA into the nucleus by promoting escape from the endosomal compartments and traffic through the host Golgi network. Mechanistically, the C-terminus of L2 possesses a cell-penetrating peptide that protudes from the host endosome, interacts with host cytoplasmic retromer cargo and thereby mediates the capsid delivery to the host trans-Golgi network. Plays a role through its interaction with host dynein in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to host PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAntigen design and domain selection to better capture neutralizing versus non-neutralizing antibody responses.\u003c\/li\u003e\n\u003cli\u003eHigh-throughput serology and antibody screening using standardized antigens and plate-based formats.\u003c\/li\u003e\n\u003cli\u003eIntegrating binding kinetics with epitope mapping to support variant-aware immunology studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eCoat plates with HPV18-L2 antigen for ELISA antibody titration (serum\/plasma).\u003c\/li\u003e\n\u003cli\u003eScreen anti-HPV18-L2 antibodies by indirect ELISA and immunoblot readouts.\u003c\/li\u003e\n\u003cli\u003eMap antigenic epitopes using HPV18-L2 fragments\/domains (in vitro binding assays).\u003c\/li\u003e\n\u003cli\u003eDevelop antigen-capture assays using HPV18-L2 as a standard (spike-in controls).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret results in the context of the biological system, assay format, and any known domain\/isoform constraints for the target.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAntigenic proteins may contain immunodominant regions; domain choice can affect assay readouts and cross-reactivity.\u003c\/li\u003e\n\u003cli\u003eInclude relevant negative controls (e.g., unrelated antigens) and dilution series to support interpretation of binding signals.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProtKB entry (P06793) — UniProt: https:\/\/www.uniprot.org\/uniprotkb\/P06793 - NCBI Gene search (L2) — NCBI: https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=L2 - PubMed search — NLM: https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HPV18-L2 - Reactome pathway browser — Reactome: https:\/\/reactome.org\/ - InterPro protein family resource — EMBL-EBI: https:\/\/www.ebi.ac.uk\/interpro\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53059243540845,"sku":"CSB-EP356939HMNc7-1MG","price":2539.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53059337355629,"sku":"CSB-EP356939HMNc7-100UG","price":595.34,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53059337388397,"sku":"CSB-EP356939HMNc7-20UG","price":315.18,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP356939HMNc7-SDS.jpg?v=1772280074","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-papillomavirus-type-18-minor-capsid-protein-l2-l2-bhp10513967","provider":"BioHippo","version":"1.0","type":"link"}