{"product_id":"recombinant-human-pd-l1-b7-h1-cd274-protein-his-tag-bhp10304513","title":"Recombinant Human PD-L1\/B7-H1\/CD274 Protein (His Tag)","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-right:8px}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eCD274; also known as B7-H1 or programmed death ligand 1 (PD-L1); is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1; CD274; B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1\/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells; PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlternative names\u003c\/strong\u003e: B7 Homolog 1;B7-H1;B7H1;CD274;PD-L1;PDCD1 Ligand 1;PDCD1L1;PDCD1LG1;PDL1;Programmed Cell Death 1 Ligand 1;Programmed Death Ligand 1\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eMammalian (HEK293) Expression\u003c\/span\u003e\n\u003cp\u003eThis recombinant protein is produced using a validated Mammalian (HEK293) expression system and supplied as lyophilized powder with C-His affinity tag. Reconstitute as directed on the Certificate of Analysis before use. Suitable for biochemical binding assays, ELISA standard curves, antibody validation, protein interaction studies, and related research applications.\u003c\/p\u003e\n\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n  \u003ctr\u003e\n\u003cth\u003eUniProt ID\u003c\/th\u003e\n\u003ctd\u003eQ9NZQ7\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eExpression System\u003c\/th\u003e\n\u003ctd\u003eMammalian (HEK293)\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eExpression Region\u003c\/th\u003e\n\u003ctd\u003ePhe19-Thr239\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eAffinity Tag\u003c\/th\u003e\n\u003ctd\u003eC-His\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eCalculated MW\u003c\/th\u003e\n\u003ctd\u003e26.3 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eObserved MW\u003c\/th\u003e\n\u003ctd\u003e34-42 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt; 95% as determined by reducing SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eEndotoxin\u003c\/th\u003e\n\u003ctd\u003e\u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 μm filtered solution of 20mM PB, 4% Mannitol, 0.02% Tween…\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eGenerally, lyophilized proteins are stable for up to 12 months when stored at -2…\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eShelf Life\u003c\/th\u003e\n\u003ctd\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003cdiv class=\"bhp-qc-badge\"\u003e✓ Purity Verified by SDS-PAGE\u003c\/div\u003e \u003cdiv class=\"bhp-qc-badge\"\u003e✓ LAL Endotoxin-Tested\u003c\/div\u003e\n\n\u003ch2\u003eFunctional Activity Data\u003c\/h2\u003e\n\u003cp\u003eImmobilized Anti-Human PDL1 mAb at 2μg\/ml (100 μl\/well)can bind Human PD-L1-His.The ED50 of Human PD-L1-6His is 5.57 ng\/ml.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cp\u003eSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Elabscience Biotechnology Co Ltd","offers":[{"title":"10 ug","offer_id":53326490861933,"sku":"PKSH033557_10μg","price":58.0,"currency_code":"USD","in_stock":true},{"title":"50 ug","offer_id":53326512488813,"sku":"PKSH033557_50μg","price":128.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ZiH0C0.png?v=1778704764","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-pd-l1-b7-h1-cd274-protein-his-tag-bhp10304513","provider":"BioHippo","version":"1.0","type":"link"}