{"product_id":"recombinant-human-probable-global-transcription-activator-snf2l2-smarca2-partial-biotinylated-bhp10510605","title":"Recombinant Human Probable global transcription activator SNF2L2 (SMARCA2), partial, Biotinylated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eRecombinant Human Probable global transcription activator SNF2L2 (SMARCA2), partial, Biotinylated is a recombinant protein reagent derived from Homo sapiens (Human) and produced in E.coli. It is commonly used to support Neuroscience research by enabling binding assays, assay development and protein–protein interaction studies in controlled in vitro settings.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eExpressed region:\u003c\/strong\u003e 700-1216aa. Region selection can focus on functional domains, improve solubility, or isolate interaction surfaces for targeted studies.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eExpression system:\u003c\/strong\u003e E.coli. Expression host can influence folding and the presence\/absence of post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eTag \/ fusion:\u003c\/strong\u003e N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. Tags can support purification and detection; evaluate potential tag effects when studying sensitive interactions.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight (reported):\u003c\/strong\u003e 107.5 kDa. Apparent size may vary with tags, processing, and gel conditions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWhen comparing results across batches or platforms, interpret signals in the context of construct design (region, tags) and expression host, especially for modification-dependent interactions.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eThe gene commonly associated with this target is \u003cstrong\u003eSMARCA2\u003c\/strong\u003e. SMARCA2 refers to a protein target that is studied across multiple biological contexts; annotations and nomenclature can vary by species and isoform. This product corresponds to the Homo sapiens (Human) sequence context, which can be important when comparing homologs or orthologs across model systems. For curated functional annotations, domains, and sequence features, consult primary databases (e.g., UniProt\/NCBI) and the recent literature for the specific organism and isoform.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eLinking protein expression or modification states to neuronal activity, synaptic plasticity, and circuit phenotypes.\u003c\/li\u003e \u003cli\u003eStudying receptor\/ligand or scaffolding interactions that influence synapse organization and signaling.\u003c\/li\u003e \u003cli\u003eDeveloping quantitative assays to track neurodegeneration- or injury-associated molecular changes.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eRelevance:\u003c\/strong\u003e Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI\/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically . Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem\/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem\/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A\/BAF53A and PHF10\/BAF45A, are exchanged for homologous alternative ACTL6B\/BAF53B and DPF1\/BAF45B or DPF3\/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal\/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth .\u003c\/p\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eAssay and standard development for immunoassays or binding-based detection methods.\u003c\/li\u003e \u003cli\u003eProtein–protein interaction studies (e.g., receptor–ligand or complex assembly) using purified components.\u003c\/li\u003e \u003cli\u003eStructure–function analysis, including domain mapping or evaluation of sequence variants.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eIn quantitative assay development, changes in binding or activity readouts are typically interpreted relative to appropriate negative\/positive controls and, where possible, orthogonal assay formats that support the same conclusion.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e \u003cli\u003eRecombinant constructs may represent a defined region (domain) rather than the full-length protein; interpret results in the context of the expressed region.\u003c\/li\u003e \u003cli\u003eTag or fusion elements can aid purification and detection but may influence binding surfaces or oligomerization; consider tag controls when relevant.\u003c\/li\u003e \u003cli\u003eSpecies and isoform differences can affect interaction partners and post-translational modifications; align experimental controls to the intended biological context.\u003c\/li\u003e \u003cli\u003eE. coli expression can limit eukaryotic post-translational modifications; for modification-dependent biology, interpret results accordingly.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry for P51531 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P51531\/entry - NCBI Gene search (SMARCA2) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SMARCA2 - PubMed search (SMARCA2) — NCBI — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SMARCA2 - RCSB PDB search (SMARCA2) — RCSB PDB — https:\/\/www.rcsb.org\/search?query=SMARCA2 - Reactome Pathway Browser — Reactome — https:\/\/reactome.org\/ --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"1 mg","offer_id":53065325183341,"sku":"CSB-EP021799HU-B-1MG","price":2174.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53065500229997,"sku":"CSB-EP021799HU-B-100UG","price":502.0,"currency_code":"USD","in_stock":true},{"title":"20 ug","offer_id":53065500262765,"sku":"CSB-EP021799HU-B-20UG","price":268.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-EP021799HU-B-SDS.jpg?v=1772476606","url":"https:\/\/www.ebiohippo.com\/products\/recombinant-human-probable-global-transcription-activator-snf2l2-smarca2-partial-biotinylated-bhp10510605","provider":"BioHippo","version":"1.0","type":"link"}